MHC Protocols / Edition 1

by Stephen H. Powis, Robert W. Vaughan

Overview

The aim of MHC Prools is to document prools that can be used for the analysis of genetic variation within the human major hisompatibility complex (MHC; HLA region). The human MHC encompasses approximately 4 million base pairs on the short arm of chromosome 6 at cytogenetic location 6p21. 3. The region is divided into three subregions. The telomeric class I region contains the genes that encode the HLA class I molecules HLA-A, -B, and -C. The centromeric class II region contains the genes encoding the HLA class II molecules HLA-DR, -DQ, and -DP. In between is the class III region, originally identified because it contains genes encoding components of the complement pathway. The entire human MHC has recently been sequenced (1) and each subregion is now known to contain many other genes, a number of which have immunological functions. The study of polymorphism within the MHC is well established, because the region contains the highly polymorphic HLA genes. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. As a result, large numbers of HLA alleles have been identified, a process that has been further driven by recent interest in HLA gene diversity in ethnic populations. The extreme genetic variation in HLA genes is believed to have been driven by the evolutionary response to infectious agents, but relatively few studies have analyzed associations between HLA genetic variation and infectious disease, which has been difficult to demonstrate.

Product Details

ISBN-13:	9780896035485
Publisher:	Springer-Verlag New York, LLC
Publication date:	09/18/2002
Series:	Methods in Molecular Biology , #210
Edition description:	2003
Pages:	340
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Databases.- HLA Informatics.- Accessing HLA Sequencing Data Through the 6ace Database.- Polymorphism in Classical and Nonclassical Hla Genes.- HLA Typing by Restriction Fragment Length Polymorphism Analysis.- PCR-Restriction Fragment Length Polymorphism Typing of Class I and II Alleles.- PCR-Sequence-Specific Oligonucleotide Probe Typing for HLA-A, -B, and -DR.- HLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide Probes.- PCR-Sequence-Specific Primer Typing of HLA Class I and Class II Alleles.- HLA Typing With Reference Strand-Mediated Conformation Analysis.- Sequencing Prools for Detection of HLA Class I Polymorphism.- HLA-E and HLA-G Typing.- Typing Alleles of HLA-DM.- Polymorphism in Non-Hla Mhc Genes.- Typing Alleles of TAP1 and TAP2.- Determining Alleles of the C2 Gene by Southern Blotting.- Complement C4 Protein and DNA Typing Methods.- Typing of Tumor Necrosis Factor Alleles.- Molecular Typing of the MHC Class I Chain-Related Gene Locus.- Microsatellites.- HLA Microsatellite Analysis.

Editorial Reviews

In this book, leading experts in molecular biology research detail reproducible methods for identifying polymorphisms in human MHC. Described in step-by-step detail to ensure robust and successful experimental results, these techniques provide DNA-based protocols for the study of polymorphism in HLA class I and II genes and in non-HLA HMC genes associated with human disease. Contributors also describe methods for accessing HLA sequence data from electronic databases designed to catalog HLA-region genes and their alleles. Each method is detailed by an investigator who has used the technique extensively. Powis is affiliated with the Center for Nephrology at Royal Free and University College Medical School, University College London. Vaughan is affiliated with South Thames Tissue Typing in the Division of Medicine at Guy's Campus, King's College, UK. Annotation c. Book News, Inc., Portland, OR