Methods of Chromatography

by Richard A. Neuhaus

Overview

The Neuhaus Science Projects Guide, Methods of Chromatography, is written for students in the junior/senior high school. This booklet describes some of the basic techniques used to separate and identify chemicals. There are excellent photographs and illustrations throughout.

Methods and topics discussed:

Paper Chromatography:-- A Simple Experiment Tells All • Chromatography • How the Method of Paper Chromatography Works • Solvents • The Spot Technique of Paper Chromatography • Materials Needed • Matching Controls • Reagents • Preparation of Unidentified Mixtures Prior to Separation • Technique of Paper Chromatography • Practice Runs with Food Dyes • Testing Unknowns • Some Hazardous Solvents to Avoid • Selecting a Solvent • Some Visualization Reagents • Quantitative Analysis.

Thin Layer Chromatography (TLC):-- How to Coat Glass Microscope Slides • TLC Kits • Solvents.

Adsorption Column Chromatography:-- Microscale Columns • Packing the Column • Sample Preparation • The Macroscale Technique • Elution • Bands • Experimental Conditions • Project Ideas • Other Resources • Sorbent Selection Table.

Gel filtration:-- Example Project

Other Systems of Chromatography:-- Electrophoresis • Other Methods

Suggested Projects:-- Variables for Further Study • A Final Note on All Techniques

References

Product Details

BN ID:	2940151134408
Publisher:	Gormley Publishing
Publication date:	11/02/2015
Sold by:	Barnes & Noble
Format:	eBook
Pages:	67
File size:	3 MB

About the Author

Richard Newhouse holds a Master of Science degree specializing in cellular and molecular biology from Cleveland State University, where he has done research in the fields of endocrinology and the aging process. In addition, he has an undergraduate teaching degree in biology from Waynesburg University, and a major in chemistry. He also holds a Master of Education degree from Rutgers University.

Mr. Newhouse has had 32 years of broad teaching experience in secondary education at all grade levels, 7-12, in the subjects of general science, biology, and chemistry. He has written numerous books on science activities – some published under his ancestral name "Neuhaus." He was a volunteer judge at regional and state level science fairs in Ohio. Many of his students won science project awards and later chose science careers.