Methods in Pharmacology: Molecular and Cellular Biology of Pharmacological Targets / Edition 1

by H. Glossmann, J. Striessnig

Overview

The current volume provides detailed experimental prools used to study plasma membrane ion channels as pharmacological targets. Coverage includes molecular and biochemical characterization of ion channels; fuctional analysis of ion channels after reconstitution, expression, or in cells; and specific methods and tools. This wealth of information will benefit academic and industrial researchers and graduate students in pharmacology, biochemistry, physiology, and biophysics.

Product Details

ISBN-13:	9781441932327
Publisher:	Springer US
Publication date:	10/29/2010
Edition description:	Softcover reprint of hardcover 1st ed. 1993
Pages:	278
Product dimensions:	7.01(w) x 10.00(h) x 0.02(d)

Table of Contents

I Molecular and Biochemical Characterization of Ion Channels.- 1 Purification and Pharmacological Analysis of the Omega-Conotoxin GVIA Receptor from Rat Brain.- 2 Molecular Characterization of the Charybdotoxin-Sensitive, High-Conductance, Calcium-Activated Potassium Channel.- 3 Characterization of the Sulfonylurea-Sensitive ATP-Modulated Potassium Channel.- II Functional Analysis of Ion Channels.- 4 Xenopus Oocytes as an Expression System for Ion Channels.- 5 Reconstitution of Muscle Calcium Channel Function in Bilayer Membranes: From the First Steps to Results.- 6 Approaches to Studying the Interaction between G-Proteins and Voltage-Dependent Ca2+ Channels.- 7 Influence of GABA on Potassium Channels in Hippocampal Neurons.- 8 Estimation of Drug Affinities for Calcium Channel Conformational States.- III Specific Methods and Tools.- 9 Production of Monoclonal Antibodies against Low-Abundance Ion-Channel-Associated Proteins in Neuronal Tissue.- 10 A Highly Porous Sodium Dodecyl Sulfate-Polyacrylamide Gel System: A Versatile Technique for Protein Structure and Function Study.- 11 Synthesis and Properties of Diazipine, a Novel Photoaffinity Probe for Calcium Channel-Linked Dihydropyridine Receptors.

Editorial Reviews

Specialists in the field of ion channels provide an in-depth view of current biochemical and electrophysiological methods, focusing on the cation-selective ion channels that are of special pharmacological interest. Discusses the molecular and biochemical characterization of ion channels, the functional analysis of ion channels (after reconstitution or expression, or in cells), and several specific methods and tools. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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