Metastasis Research Protocols / Edition 1

by Susan A. Brooks, Udo Schumacher

Overview

In Volume I, Analysis of Cells and Tissues, we presented a range of prools aimed at mapping and analyzing the expression of various molecules of pot- tial interest in metastasis research and for examining their production at the genetic level. In this second volume of metastasis research prools, we move to the level of living cells and tissues and present methodologies applicable to examining metastatic behavior in vitro and in whole animal models. The methods described in the first section of this volume concentrate on the separation of cell lines with high and low metastatic potential, including the genetic modification of cell lines. The assay systems to test defined aspects of the metastatic cascade are then described in Part II and include cell migration assays, assays for matrix degrading enzymes, basement membrane degrading assays, adhesion assays, and assays of angiogenesis. The role of the specific elements of the metastatic cascade assayed in each of these systems in turn must of course be put into perspective relative to their roles in entire living organisms.

Product Details

ISBN-13:	9781617370991
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/09/2010
Series:	Methods in Molecular Medicine , #58
Edition description:	Softcover reprint of hardcover 1st ed. 2001
Pages:	310
Product dimensions:	5.98(w) x 9.02(h) x 0.03(d)

About the Author

Brooks, Susan A.; Schumacher, Udo

Table of Contents

Part I. Selection/Segregation of Cell Populations for In vitro and In Vivo Assays of Metastatic Behavior Cell Separations by Flow Cytometry Derek Davies Immunomagnetic Cell Separation Catherine Clarke and Susan Davies Genetic Modification of Cell Lines to Enhance Their Metastatic Capability Daniel McWilliams and Hilary Collins Part II. In Vitro Assays of Metastatic Behavior Cell Aggregation Assays, Tom Boterberg Marc E. Bracke, Erik A. Bruyneel, and Marc M. Mareel Cell Migration and the Boyden Chamber Nicholas S. Brown and Roy Bicknell Quantification of Cell Motility: Gold Colloidal Phagokinetic Track Assay and Wound Healing Assay Yasufumi Niinaka, Arayo Haga, and Avraham Raz In Vitro Invasion Assay Using Matrigel® Debbie M. S. Hall and Susan A. Brooks Membrane Invastion Culture System Mary J. C. Hendrix, Elisabeth A. Seftor, and Richard E. B. Seftor Collagen Invasion Assay Marc E. Bracke, Tom Boterberg, Erik A. Bruyneel, and Marc M. Mareel Chick Heart Invasion Assay Marc E. Bracke, Tom Boterberg, and Marc M. Mareel Adhesion of Tumor Cells to Matrices and Endothelium G. Ed Rainger Assessment of Angiogenic Factors: The Chick Chorioallantoic Membrane Assay Adam Jones, Chisato Fujiyama, Stephen Hague, and Roy Bicknell Methods to Evaluate the Formation and Stabilization of Blood Vessels and Their Role in Tumor Growth and Metastasis Nerbil Kilic and Süleyman Ergün Galectin-3 Binding and Metastasis Pratima Nangia-Makker, Yuichiro Honjo, and Avraham Raz Part III. Animal Models of Metastasis Basic Principles for the Study of Metastasis Using Animal Models Suzanne A. Eccles The Chick Embryo Metastasis Model Eric Petitclerc and Peter C. Brooks Gene Transfection for Metastasis ResearchUsing Animal Models Ruth J. Muschel and Jim Hua Theoretical Considerations in Using Animal Models of Metastastis and Brief Methodology for In Vivo Colorectal Cancer Models in SCID and Nude Mice Sue A. Watson and Teresa M. Morris Xenograft Models in Immunodeficient Animals: I. Nude Mice: Spontaneous and Experimental Metastatis Models Janet E. Price Xenograft Models in Immunodeficient Animals: II. Use of SCID Mice in Metastasis Research: Breast and Colon Cancer Models of Metastasis Udo Schumacher and Susan A. Brooks Syngeneic Murine Metastasis Model: B16 Melanoma Raffaella Giavazzi and Angela Garofalo Transgenic Animal Models Chantale Guy and Gizela Cordosa Clinically Accurate Orthotopic Mouse Models of Cancer Robert M. Hoffman Dissection of Tumor and Host Cells from Metastasized Organs for Testing Gene Expression Directly Ex Vivo Marian Rocha, Volker Schirrmacher, and Victor Umansky Green Fluorescent Protein for Metastastis Research Robert M. Hoffman

Editorial Reviews

This book is a valuable addition to the literature as it brings together key people in the field of cancer metastasis to provide practical step-by-step prools. . . This book is written in such as way that it would be useful to beginning students and to anyone curious about the methods that are available to study the key steps in cancer metastasis. This book would also be useful to seasoned researchers as it brings together a number of different methodologies in one easy-to-read book. . . This is a very practical book covering areas of research in cancer invasion and metastasis. The greatest strength of the book is in collecting the different prools that are useful in studying cellular behavior in one easy-to-read book.."-Weighted Numerical Score: 91 - 4 Stars!-Doody's Health Sciences Book Review Journal

"These volumes should be of interest for all medical and academic libraries where cancer research is performed." - E-Streams

From the Publisher

4 Stars! from Doody