Liposomes: Methods and Protocols

by Gerard G.M. D'Souza (Editor), Hongwei Zhang (Editor)

Overview

This third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.

Product Details

ISBN-13:	9781071629567
Publisher:	Springer US
Publication date:	02/14/2024
Series:	Methods in Molecular Biology , #2622
Edition description:	3rd ed. 2023
Pages:	305
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Liposomes Came First: The Early History of Liposomology.- Thin Film Hydration followed by Extrusion Method for Liposome Preparation.- Preparation of DRV Liposomes.- Method of Simultaneous Analysis of Liposome Components by HPTLC/FID.- Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale.- Liposome Formulations of Hydrophobic Drugs.- A “Dock and Lock” Approach to Preparation of Targeted Liposomes.- Coupling of Ligands to the Liposome Surface by Click Chemistry.- Elastic Liposomes for Topical and Transdermal Drug Delivery.- Determination of the Sub-Cellular Distribution of Liposomes using Confocal Microscopy.- Environmental Scanning Electron Microscope Imaging of Vesicle Systems.- Liposome Biodistribution via Europium Complexes.- Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Preformed Liposomes for Diagnostic Imaging and Radionuclide Therapy.- Gadolinium-Loaded Polychelating Polymer-Containing Tumor-Targeted Liposomes.- Serum-Stable, Long-Circulating, pH-Sensitive PEGylated Liposomes.- Long-Circulating, pH-Sensitive Liposomes.- Anionic pH-Sensitive Lipoplexes.- Fluorometric Analysis of Individual Cationic Lipid-DNA Complexes.- Preparation and Physical Characterization of DNA Binding Cationic Liposomes.- Fluorescence Resonance Energy Transfer (FRET) Based Analysis of Lipoplexes.- Targeted Magnetic Liposomes Loaded with Doxorubicin.- Stable Discoidal Bicelles - A Platform of Lipid Nanocarriers for Cellular Delivery.