Ion Channels of Excitable Cells

by Toshio Narahashi (Editor)

Overview

Because of the highly significant and widely recognized roles of ion channels in physiology, pathophysiology, pharmacology, and toxicology, the term ion channel has now become a household word in the biomedical sciences. This volume covers preparations and techniques for the study of various ion channels. Both voltage-gated and ligand-gated ion channels of neurons, axons, and cardiac and smooth muscles are covered. It includes not only patch clamp techniques but molecular biology and imaging techniques as well.

• Comprehensive protocols included for the study of: Ion channels using patch-clamp, molecular biology, and imaging techniques
• Role of ion channels in physiology, pathophysiology, pharmacology, and toxicology
• Specific ion channels of specific tissues

Product Details

ISBN-13:	9781483288338
Publisher:	Elsevier Science
Publication date:	10/22/2013
Series:	ISSN , #19
Sold by:	Barnes & Noble
Format:	eBook
Pages:	387
File size:	7 MB

About the Author

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and Obstetrics and Gynecology at Oregon Health and Science University and Senior Scientist of the Oregon National Primate Research Center (ONPRC). He served for twelve years as Special Assistant to the President and Associate Director of the ONPRC. After receiving a B.S. degree and teaching certification from the University of Michigan (1971), a M.S. from North Carolina State University (1973), and a Ph.D. degree from Baylor College of Medicine (1976), Conn did a fellowship at the NIH, then joined the faculty in the Department of Pharmacology, Duke University Medical Center where he was promoted to Associate Professor in 1982. In 1984, he became Professor and Head of Pharmacology at the University of Iowa College of Medicine, a position he held for eleven years. Conn is known for his research in the area of the cellular and molecular basis of action of gonadotropin releasing hormone action in the pituitary and therapeutic approaches that restore misfolded proteins to function. His work has led to drugs that have benefitted humans and animals. Most recently, he has identified a new class of drugs, pharmacoperones, which act by regulating the intracellular trafficking of receptors, enzymes and ion channels. He has authored or co-authored over 350 publications in this area and written or edited over 200 books, including texts in neurosciences, molecular biology and endocrinology. Conn has served as the editor of many professional journals and book series (Endocrinology, Journal of Clinical Endocrinology and Metabolism, Endocrine, Methods, Progress in Molecular Biology and Translational Science and Contemporary Endocrinology). Conn served on the National Board of Medical Examiners, including two years as chairman of the reproduction and endocrinology committee. The work of his laboratory has been recognized with a MERIT award from the NIH, the J.J. Abel Award of the American Society for Pharmacology and Experimental Therapeutics, the Weitzman, Oppenheimer and Ingbar Awards of the Endocrine Society, the National Science Medal of Mexico (the Miguel Aleman Prize) and the Stevenson Award of Canada. He is the recipient of the Oregon State Award for Discovery, the Media Award of the American College of Neuropsychopharmacology and was named a distinguished Alumnus of Baylor College of Medicine in 2012. Conn is a previous member of Council for the American Society for Cell Biology and the Endocrine Society and is a prior President of the Endocrine Society, during which time he founded the Hormone Foundation and worked with political leadership to heighten the public’s awareness of diabetes. Conn’s students and fellows have gone on to become leaders in industry and academia. He is an elected member of the Mexican Institute of Medicine and a fellow of the American Association for the Advancement of Science. He is the co-author of The Animal Research War (2008) and many articles for the public and academic community on the value of animal research and the dangers posed by animal extremism. His op/eds have appeared in The Washington Post, The LA Times, The Wall Street Journal, the Des Moines Register, and elsewhere. Conn consults with organizations that are influenced by animal extremism and with universities and companies facing challenges from these groups.

Table of Contents

Neuronal Voltage-Gated Ion Channels: Electrophysiology:F.N. Quandt, Recording Sodium and Potassuim Currents from Neuroblastoma Cells.M.L. Roy and T. Narahashi, Sodium Channels of Rat Dorsal Root Ganglion Neurons.D.J. Surmeier, C.J. Wilson, and J. Eberwine, Patch-Clamp Techniques for Studying Potassium Currents in Mammalian Brain Neurons.D.G. Puro, Calcium Channels of Human Retinal Glial Cells.J.G. Starkus and M.D. Rayner, Axial-Wire Voltage Clamping in Crayfish Medial Giant Axons.S.Y. Chiu and P. Shrager, Biophysical Studies of Ion Channels in Glial Cells and Axons of Myelinated Nerves.Ligand-Gated Ion Channels: Electrophysiology:J.G. Montes, M. Alkondon, E.F.R. Pereira, N.G. Castro, and E. Albuquerque, Electrophysiological Methods for the Study of Neuronal Nicotinic Acetylcholine Receptor Ion Channels.N. Akaike and N. Harata, GABAA> Receptor-Chloride Channel Complex.Cardiac and Smooth Muscle Voltage-Gated Ion Channels: Electrophysiology:M.F. Sheets and R.E. Ten Eick, Whole-Cell Voltage-Clamp of Cardiac Sodium Current.R.S. Kass and R. Bangalore, Measurement of Dihydropyridine Modulation of Cardiac L-Type Calcium Channels: Molecular and Cellular Implications.M.C. Sanguinetti, Potassium Channels of Cardiac Myocytes.J.M. Farley, Airway Smooth Muscle Ion Channels.Molecular Biology and Electrophysiology:J.A. Drewe, H.A. Hartmann, and G.E. Kirsch, Potassium Channels in Mammalian Brain: A Molecular Approach.M.W. Quick and H.A. Lester, Methods for Expression of Excitability Proteins in Xenopus Oocytes.Imaging and Electrophysiology:A.B. MacDermott, V. Henzi, and D.B. Reichling, Characterization of Glutamate Receptor Function Using Calcium Photometry and Imaging.J.F. MacDonald, L.-Y. Wang, and M.W. Salter, Phosphorylation of Glutamate Receptors Using Electrophysiology Techniques.B.W. Hicks and K.J. Angelides, Imaging Ion Channel Dynamics in Living Neurons by Fluorescence Microscopy.S.R. Glaum, S. Alford, D.J. Rossi, G.L. Collingridge, and N.T. Salter, Whole-Cell Patch Recording with Simultaneous Measurement of Intracellular Calcium Concentration in Mammalian Brain Slices in Vitro.Lipid Bilayers:A. Grove, A.F. Ferrer-Montiel, and M. Montal, Reconstitution of Channel Proteins from Excitable Cells in Lipid Bilayers: Authentic and Designed Proteins.Index.