Imaging Flow Cytometry: Methods and Protocols

by Natasha S. Barteneva, Ivan A. Vorobjev

Overview

This detailed volume for the first time explores techniques and prools involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing prools for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC prools for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Imaging Flow Cytometry: Methods and Prools willbe a critical source for all laboratories seeking to implement IFC in their research studies.

Product Details

ISBN-13:	9781493949014
Publisher:	Springer New York
Publication date:	08/23/2016
Series:	Methods in Molecular Biology , #1389
Edition description:	Softcover reprint of the original 1st ed. 2016
Pages:	295
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Quantitative Functional Morphology by Imaging Flow Cytometry.- Principles of Amnis Imaging Flow Cytometry.- Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch.- Applications of Imaging Flow Cytometry for Microalgae.- The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry.- Quantitation of Chromosome Damage by Imaging Flow Cytometry.- Fluorescent In Situ Hybridization In Suspension by Imaging Flow Cytometry.- Analysis of Nucleo-Cytoplasmic Protein Shuttling by Imaging Flow Cytometry.- Using Image-Based Flow Cytometry with a FISH-Based FlowRNA Assay to Simultaneously Detect Intracellular TNF-α Protein and mRNA in Monocytes Following LPS Stimulation.- Multiparametric Characterization of Human T-Cell Immune Synapses by InFlow Microscopy.- Studying T Cells N-Glycosylation by Imaging Flow Cytometry.- Assessment of Granulocyte Subset Activation: New Information from Image-Based Flow Cytometry.- Using Image-Based Flow Cytometryto Assess Monocyte Oxidized LDL Phagocytosis Capacity.- Screening for Drugs Against the Plasmodium falciparum Digestive Vacuole by Imaging Flow Cytometry.- Accurate Assessment of Cell Death by Imaging Flow Cytometry.- Temporal Heterogeneity in Apoptosis Determined by Imaging Flow Cytometry.- FlowCAM: Quantification and Classification of Phytoplankton by Imaging Flow Cytometry.- Detection and Characterization of Rare Circulating Endothelial Cells by Imaging Flow Cytometry.- Imaging Flow Cy

tometric Analysis of Primary Bone Marrow Megakaryocytes.- Sickle Cell Imaging Flow Cytometry Assay (SIFCA).

Editorial Reviews

The purpose is to present to the scientific community the compendium of recently developed imaging flow cytometry techniques and prools. The authors are successful in achieving these worthy objectives. Intended for the scientific community, this book will be very useful for students, graduate students, and scientists interested in learning about imaging flow cytometry and its applications. … This book for the first time explores the techniques and prools involving quantitative imaging flow cytometry in a fascinating manner.” (Omer Iqbal, Doody's Book Reviews, September, 2016)

From the Publisher

Reviewer: Omer Iqbal, MD, FACC, FESC (Loyola University Medical Center)
Description: This unique book covers the techniques and protocols involving quantitative imaging flow cytometry (IFC) crucial for analyzing cells and cellular clusters and populations with an ample number of high quality illustrations.
Purpose: The purpose is to present to the scientific community the compendium of recently developed imaging flow cytometry techniques and protocols. The authors are successful in achieving these worthy objectives.
Audience: Intended for the scientific community, this book will be very useful for students, graduate students, and scientists interested in learning about imaging flow cytometry and its applications.
Features: Part I of the book's five parts provides a basic introduction to imaging flow cytometry and its different variants. Part II describes the use of IFC in the detection of nuclei and nucleic acids and FISH techniques. Part III covers IFC in immune response and drug screening, including a detailed analysis of apoptosis. Part IV deals with IFC in studies of cell death and apoptosis. The final part covers imaging cytometry in cell classification and morphological analysis and the identification of rare cells. The numerous high-quality illustrations make a complex subject easier to understand.
Assessment: This book for the first time explores the techniques and protocols involving quantitative imaging flow cytometry in a fascinating manner. The scientific community comprised of students, graduate students, researchers, and scientists will find this book quite useful.

Doody's Review Service