Histochemistry of Single Molecules: Methods and Protocols

Histochemistry of Single Molecules: Methods and Protocols

Histochemistry of Single Molecules: Methods and Protocols

Histochemistry of Single Molecules: Methods and Protocols

Hardcover(2nd ed. 2023)

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Overview

This detailed volume explores numerous hishemical techniques through a series of lab-tested prools for the detection of specific molecules or metabolic processes, both at light and electron microscopy. More in detail, the book is divided into six sections covering a variety of chemical targets. It begins with a section on vital hishemistry and continues with chapters on hishemistry as it relates to lectins, proteins, lipids, DNA and RNA, as well as plants. The volume also contains four overview chapters on vital hishemistry, lectin hishemistry, and DNA fluorochromes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Hishemistry of Single Molecules: Methods and Prools aims to effectively help scientists in very different research fields to elucidate biological issues though a unique approach to molecular biology in situ.

Product Details

ISBN-13: 9781071626740
Publisher: Springer US
Publication date: 09/25/2022
Series: Methods in Molecular Biology , #2566
Edition description: 2nd ed. 2023
Pages: 357
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Single Cell Cyhemistry Illustrated by the Demonstration of Glucose-6-Phosphate Dehydrogenase Deficiency in Erythrocytes.- Autofluorescence Spectroscopy for Monitoring Metabolism in Animal Cells and Tissues.- Enzyme-Hishemistry Technique for Visualizing the Dipeptidyl-Peptidase IV (DPP-IV) Activity in the Liver Biliary Tree.- Hishemical Demonstration of Tripeptidyl Aminopeptidase I.- Enzyme Hishemistry for Functional Histology in Invertebrates.- Lectin Hishemistry: Historical Perspectives, State of the Art, and the Future.- Isolation of Viable Glycosylation-Specific Cell Populations for Further In Vitro or In Vivo Analysis Using Lectin-Coated Magnetic Beads.- Lectin Hishemistry for Metastasizing and Non-Metastasizing Cancer Cells.- The Use of Lectin Hishemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium.- Heat-Induced Antigen Retrieval in Immunohishemistry: Mechanisms and Applications.- Detecting Neuronal Differentiation Markers in Newborn Cells of the Adult Brain.- Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration.- Immunohishemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin Fixed and Paraffin Embedded Tissue.- Tissue Fixation and Processing for the Histological Identification of Lipids.- Staining Methods for Normal and Regenerative Myelin in the Nervous System.- Nile Red Staining of Neutral Lipids in Yeast.- Staining of Lipid Droplets with Monodansylpentane.- Fluorochromes for DNA Staining and Quantitation.- Osmium Ammine for Staining DNA at Electron Microscopy.- DNA Labeling at Electron Microscopy.- Visualizing RNA at Electron Microscopy by Terbium Citrate.- Two-Tailed Comet Assay (2T-Comet): Simultaneous Detection of DNA Single and Double Strand Breaks.- Detection of Endogenous Nuclear Proteins in Plant Cells: Localizing Nuclear Matrix Constituent Proteins (NMCPs), the Plant Analogues of Lamins.- Hishemical Analysis of Plant Secretory Structures.- A Hishemical Technique for the Detection of Annonaceous Acetogenins.

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