High Performance Liquid Chromatography: Principles and Methods in Biotechnology / Edition 1

by Elena D. Katz

Overview

Analytical techniques based on separation processes, such aschromatography and electrophoresis, are finding a growing range ofapplications in chemical, pharmaceutical and clinical laboratories.The Wiley Separation Science Series provides the analyst in theselaboratories with well focused books covering individualtechniques, so that they can be applied more efficiently andeffectively to contemporary analytical problems. In biotechnology,biochemistry and molecular biology, the characterization andanalysis of biomolecules such as proteins, oligosaccharides andnucleic acids are of great importance. High performance liquidchromatography (HPLC) has become one of the key analytical tools inthese fields, providing rapid purification and quantitativeanalysis of biomolecules.

High Performance Liquid Chromatography: Principles and Methods inBiotechnology covers the most important theoretical and practicalaspects of the application of HPLC to the biosciences, includingsample preparation, column selection, detection of biomolecules,preparative HPLC, and the use of HPLC for specific biomolecules.Finally, the technique is compared to alternative bioseparationmethods. The ten chapters are written by researchers active in thebiotechnology field. High Performance Liquid Chromatography:Principles and Methods in Biotechnology will be of great practicaluse to molecular biologists, biochemists and biotechnologists whouse HPLC to purify and quantify biomolecules. It will also be ofinterest to those carrying out protein and DNA characterization andanalysis.

Product Details

ISBN-13:	9780471934448
Publisher:	Wiley
Publication date:	04/11/1996
Series:	Separation Science Series , #5
Pages:	536
Product dimensions:	6.28(w) x 10.28(h) x 1.44(d)

About the Author

Elena D. Katz is the author of High Performance Liquid Chromatography: Principles and Methods in Biotechnology, published by Wiley.

Table of Contents

Introduction to Chromatography in Biotechnology (C. Simpson).

The Principles of Separation by High Performance LiquidChromatography (R. Scott).

Column Selection in High Performance Liquid Chromatography (R.Eksteen).

Detection and Identification in Biochromatography (I. Krull, etal.).

Sample Preparation (A. Rizzi & I. Maurer-Fogy).

The Preparative HPLC of Biomolecules (G. Cox).

The Application of HPLC for Nucleic Acid Analysis (J. Wages &E. Katz).

High-pH Anion Exchange Chromatography of Recombinant GlycoproteinGlycans (R. Townsend).

The Application of HPLC for Proteins (K. Benedek).

Alternative Bioseparation Techniques (P. Righetti, et al.).

Index.

Editorial Reviews

For experienced analysts working in chemical, pharmaceutical, and clinical laboratories, explains in detail one of the key tools used to characterize and analyze biomolecules such as proteins, oligosaccharides, and nucleic acids. Covers both theoretical and practical aspects, including sample preparation, column selection, detection of biomolecules, preparative HPLC, and its use for specific biomolecules. Also compares the technique to other separation methods used in biotechnology, biochemistry, and molecular biology. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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