Handbook of HLA Typing Techniques

by Kam M. Hui, Jeffrey L. Bidwell

Overview

This book presents an authoritative collection of HLA phenotyping and genotyping techniques to be used at the bench level and as a reference. It includes detailed methodologies, notes on the interpretation of tests, reference material, and appendices.

Product Details

ISBN-13:	9781000153392
Publisher:	CRC Press
Publication date:	10/07/2020
Sold by:	Barnes & Noble
Format:	eBook
Pages:	430
File size:	21 MB
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About the Author

Kam M. Hui (Nat'l University/Singapore) (Author) , Jeffrey L. Bidwell (University of Bristol, United Kingdom) (Author)

Table of Contents

Introduction 1. PCR-RFLP 2. HLA-DR and -DQ Typing by DNA-RFLP Analysis 3. Rapid HLA-DR-Dw and DP Matching by PCR Fingerprinting and Related DNA Heteroduplex Technologies 4. A Comprehensive HLA-DRB, -DQB, and -DPB Oligotyping Procedure by Hybridization with Sequence-Specific Oligonucleotide Probes 5. HLA-DR Typing by Polymerase Chain Reaction Amplification with Sequence-Specific Primers (PCR-SSP) 6. Serological Typing of HLA-A, -B, and -C Antigens 7. HLA-DR and -DQ Serotyping 8. Definition of HLA-Dw Determinants Using Homozygous Typing Cells and the Mixed Lymphocyte Culture 9. Definition of HLA-Dw and HLA-DPw Determinants by the Primed Lymphocyte Test