Fundamentals of Cell Immobilisation Biotechnology / Edition 1

by Viktor Nedovic, Ronnie Willaert

Overview

Cell Immobilisation Biotechnology Biotechnology is divided into two volumes. The first volume is dedicated to fundamental aspects of cell immobilisation while the second volume deals with the diverse applications of this technology.

The first volume, Fundamentals of Cell Immobilisation Biotechnology, comprises 26 chapters arranged into four parts:

• Materials for cell immobilisation/encapsulation,
• Methods and technologies for cell immobilisation/encapsulation,
• Carrier characterisation and bioreactor design, and
• Physiology of immobilised cells: techniques and mathematical modelling.

Product Details

ISBN-13:	9789048165346
Publisher:	Springer Netherlands
Publication date:	12/03/2010
Series:	Focus on Biotechnology , #8
Edition description:	Softcover reprint of hardcover 1st ed. 2004
Pages:	555
Product dimensions:	6.30(w) x 9.45(h) x 0.05(d)

Table of Contents

1 Materials for Cell Immobilisation/Encapsulation.- Biomaterials for cell immobilization: A look at carrier design.- Alginate as a carrier for cell immobilisation.- Entrapment in LentiKats®: Encapsulation of various biocatalysts — bacteria, fungi, yeast or enzymes into polyvinyl alcohol based hydrogel particles.- Starches as encapsulation materials.- Proteins: versatile materials for encapsulation.- Polyelectrolyte complexes for microcapsule formation.- Pre-formed carriers for cell immobilisation.- Microcarriers for animal cell culture.- 2 Methods and Technologies for Cell Immobilisation/Encapsulation.- Microcapsule formulation and formation.- Liquid core caspules for applications in biotechnology.- Direct compression — novel method for encapsulation of probiotic cells.- Cell immobilisation in pre-formed porous matrices.- Whole cell immobilization in chopped hollow fibres.- Use of vibration technology for jet break-up for encapsulation of cells and liquids in monodisperse microcapsules.- Immobilization of cells and enzymes using electrostatic droplet generation.- The JetCutter technology.- Industrial scale encapsulation of cells using emulsification/dispersion technologies.- Atomisation techniques for immobilisation of cells in micro gel beads.- Spray coating and drying processes.- 3 Carrier Characterisation and Bioreactor Design.- Diffusive mass transfer in immobilised cell systems.- Characterization of microcapsules.- Immobilised cell bioreactors.- 4 Physiology of Immobilised Cells: Experimental Characterisation and Mathematical Modelling.- Applications of NMR spectroscopy and imaging to the study of immobilised cell physiology.- Physiology of immobilised microbial cells.- Modelling immobilised-cell processes: Application to integrated nitrogen removal withco-immobilised microorganisms.- Biofilm modelling.

Editorial Reviews

From the reviews:

"Many volumes have been produced describing what can be done using immobilised cells, but there are fewer that bring together information on the various fundamental aspects of cell immobilisation. This book fills that need more than adequately, providing information both for experts and for newcomers to the field, and should be available in the library of any institution involved in biotechnology. … The book is pleasingly produced and the … chapters are easy to read without in any way trivialising their subjects." (Chris Bucke, Journal of Chemical Technology and Biotechnology, Issue 81, 2006)

From the Publisher

2 Stars from Doody

Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: This book provides a compendium of research-oriented chapters on cell immobilization biotechnology. Major sections address materials and methods, bioreactor design, and cell physiology. The editors state that they have tried to collate the extensive literature on cell immobilization/encapsulation biotechnology into a comprehensive reference work. The book includes 26 chapters on subjects ranging from "micro-carriers for animal cell culture" to "immobilization of cells and enzymes using electrostatic droplet generation". The principal focus of each chapter is on chemistry, physics, biochemistry, and biophysics.
Purpose: The goal of the editors is to provide investigators with the background information and key references necessary to develop new technology in this field. Clearly, the ability to successfully confine enzymes and viable cells within carriers for delivery and distribution has profound implications for many scientific disciplines, including regenerative medicine, biologics production, industry, and agriculture. The contributions are well-written and the references are up-to-date. The authors of each chapter highlight their unique contributions to the field.
Audience: This book is written for basic science and clinical researchers in academia and industry, who are interested in cell encapsulation methodology. Graduate students, medical students, research fellows, and established investigators will read this book to broaden their horizons and gain access to the literature. The authors are experts in their field. The writing is technical and assumes extensive familiarity with the subject matter.
Features: This hard cover book represents the first of two volumes. The second volume will concentrate on the application of cell immobilization technology. The volume is carefully edited and readable. The chapters include line drawings, photomicrographs, and an extensive bibliography. The diversity of topics will capture the imagination of those working in this field.
Assessment: The book provides a wealth of information. Each chapter approaches the problem of cell immobilization/encapsulation from a different perspective. One criticism is the lack of attention to cell-matrix adhesion. I would have preferred to see a more complete discussion of this problem, with examples of interesting solutions. For example, carriers can be modified with recognition tags to facilitate the retention and differentiation of specific types of cells. The editors also did not discuss the novel use of printers to deliver biological reagents and cells in defined spatial configurations. Nonetheless, the wide range of topics presented makes this book an important contribution to the literature, and will fuel the imagination of those interested in pursuing translational research.

Doody's Review Service