Fluorescence Imaging and Biological Quantification / Edition 1

by Raquel Seruca, Jasjit S. Suri, J. Miquel Sanches

Overview

This comprehensive reference work details the latest developments in fluorescence imaging and related biological quantification. It explores the most recent techniques in this imaging technology through the utilization and incorporation of quantification analysis which makes this book unique. It also covers super resolution microscopy with the introduction of 3D imaging and high resolution fluorescence. Many of the chapter authors are world class experts in this medical imaging technology.

Product Details

ISBN-13:	9780367874155
Publisher:	CRC Press
Publication date:	12/12/2019
Pages:	358
Product dimensions:	7.00(w) x 10.00(h) x (d)

About the Author

Raquel Seruca, MD, PhD, is a Professor at Medical Faculty of Porto, vice-president of Ipatimup and Coordinator of the Cancer research program at i3s, both located in Porto, Portugal. Dr. Seruca is an expert in oncobiology with a specific interest in the role of adhesion molecules in cancer and molecular genetics of gastrointestinal cancer. She has more than 200 publications in international peer-review journals, with an h-index of 52, and several national and international awards by her relevant contributions for science and society.

Jasjit S. Suri, PhD, MBA, Fellow AIMBE, is an innovator, visionary scientist, and an internationally known world leader. Dr. Suri was crowned with the Director General’s Gold medal in 1980 and elected as a Fellow of American Institute of Medical and Biological Engineering (AIMBE), awarded by National Academy of Sciences in Washington DC in 2004. Dr. Suri has held executive positions, been the Chairman of the IEEE Denver section, and won over 50 awards during his career.

João M. Sanches, PhD, is a Professor at the Bioengineering Department (DBE) of the Instituto Superior Técnico (IST), Univers. of Lisbon, and Member of the board of directors of the Institute for Systems and Robotics (ISR/IST). Professor Sanches has about 150 international publications in Biomedical Engineering in statistical signal and image processing methods for biological and medical diagnosis. He is senior member of the IEEE Engineering in Medicine and Biology Society and Member of the Bio Imaging and Signal Processing Technical Committee (BISP-TC) of the IEEE Signal Processing Society.

Table of Contents

Preface xi

Acknowledgments xiii

Editors xv

Contributors xix

Introduction xxvii

Section I Advanced Methods of Analysis

1 Confocal microscopy in the life sciences Miguel Aroso M. Gomez-Lazaro 3

2 Imaging flow cytometry for quantification of cellular parameters Catarina R. Almeida 25

3 Live-cell imaging: Seeing is believing Patrícia Castro Patricia Carneiro 39

4 Atomic force microscopy: A tool for imaging, manipulating biomolecules, and quantifying single-molecule interactions in biological systems Catarina S. Lopes Filomena A. Carvalho Nuno C. Santos 49

5 Confocal Raman microscopy: Imaging the chemistry M. Gomez-Lazaro A. Freitas C.C. Ribeiro 65

6 Tracking cancer: In vivo imaging techniques Patricia Carvalho André Roma Sérgia Velho 85

7 Quantum dots: Concepts, imaging, and therapeutic applications in cancer Maria Sofia Fernandes Anabeia Ferro Patrícia Carneiro Raquel Seruca João M. Sanches 105

8 Selecting the appropriate in situ proximity ligation assay protocol Doroteya Raykova Linda Arngården Axel Klaesson Johan Heldin Ola Söderberg 119

9 Cell detection and joint shape tracking using local image filters Tiago Esteves Pedro Quelhas 133

Section II Molecular and Cellular Applications

10 Studying subcellular signaling events in living microglial cells using fluorescence resonance energy transfer-based nanosensors Renato Socodato Camila C. Portugal Paula Sampaio João B. Relvas 165

11 Membrane trafficking under the microscope, what new imaging technologies have brought to light Julia Fernandez-Rodriguez Tommy Nilsson 181

22 Illuminating the cycle of life Anabela Ferro Patrícia Carneiro Maria Sofia Fernandes Tania Mestre Ivan Sahumbaiev João M. Sanches Raquel Seruca 201

13 Methods for the visualization of circadian rhythms: From molecules to organisms Rukeia El-Athman Jeannine Mazuch Luise Fuhr Mónica Abreu Nikolai Genov Angela Relógio 231

14 Measurement of lysosomal ion homeostasis by fluorescence microscopy Benjamin König Lisa von Kleist Tobias Stauber 259

15 Capturing quantitative features of protein expression from in situ fluorescence microscopic images of cancer cell populations Joana Figueiredo Ana Sofia Ribeiro Tânia Mestre Sofia Esménio Martina Fonseca Joana Paredes Raquel Seruca João M. Sanches 279

16 Cancer cell invadopodia: Visualization and quantification tools Angela Margarida Costa Maria José Oliveira 299

Index 317

Editorial Reviews

"Microscopy techniques have brought significant improvements and novel implementations to several fields in the past few years. This compendium offers a fresh review of several techniques (fluorescence confocal, Raman and AFM) and their applications to life sciences; it will be useful to the experienced researcher as well as to the student approaching the use and applications of microscopy techniques."
— Beniamino Barbieri, ISS, Champaign, Illinois, USA

From the Publisher