Enzymes: Biochemistry, Biotechnology, Clinical Chemistry

by T Palmer, P L Bonner

Overview

In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.

• Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subject
• Discusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purification
• Includes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy

Product Details

ISBN-13:	9780857099921
Publisher:	Elsevier Science
Publication date:	04/04/2007
Sold by:	Barnes & Noble
Format:	eBook
Pages:	440
File size:	9 MB

Table of Contents

Part 1: Structure and Function of Enzymes 1. An Introduction to Enzymes2. The Structure of Proteins3. The Biosynthesis and Properties of Proteins4. Specificity of Enzyme Action5. Monomeric and Oligomeric Enzymes

Part 2: Kinetic and Chemical Mechanisms of Enzyme-Catalysed Reactions 6. An Introduction to Bioenergetics, Catalysis and Kinetics7. Kinetics of Single-Substrate Enzyme-Catalysed Reactions8. Enzyme Inhibition9. Kinetics of Multi-Substrate Enzyme-Catalysed Reactions10. The Investigation of Active Site Structure11. The chemical nature of enzyme catalysis12. The Binding of Ligands to Proteins13. Sigmoidal Kinetics and Allosteric Enzymes14. The Significance of Sigmoidal Behaviour

Part 3: Application of Enzymology15. Investigation of Enzymes in Biological Preparations16. Extraction and Purification of Enzymes17. Enzymes as Analytical Reagents18. Instrumental Techniques Available for Use in Enzymatic Analysis19. Applications of Enzymatic Analysis in Medicine, Forensic Science and Industry 20. Biotechnological Applications of Enzymes21. Genomics, Proteomics and Bioinformatics

Editorial Reviews

Review of the first edition: "Essential. ...explains the structure and function of enzymes. …every chapter tells of an advance in understanding proteins or their interactions. I will definitely use this book." —Chemistry and Industry (Professor Carole Hackney, Keele University, UK)

"Outstandingly written, updated on molecular biology and bioinformatics, with particularly good treatment of steady-state enzyme kinetics and analytical applications. For undergraduates in biochemistry, biological and analytical sciences, and medicine." —Natural Products Report (Royal Society of Chemistry)

"An excellent book, warmly recommended. —Trends in Biotechnology

From the Publisher