Enzyme Stabilization and Immobilization: Methods and Protocols / Edition 2

by Shelley D. Minteer

Overview

This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions).

Product Details

ISBN-13:	9781493982196
Publisher:	Springer New York
Publication date:	07/10/2018
Series:	Methods in Molecular Biology , #1504
Edition description:	Softcover reprint of the original 2nd ed. 2017
Pages:	234
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Introduction to the field of enzyme immobilization and stabilization.- Stabilization of enzymes through encapsulation in liposomes.- Micellar enzymology for thermal, pH, and solvent stability.- Lipase activation and stabilization in room temperature ionic liquids.- Nanoporous silica glass for the immobilization of interactive enzyme systems.- Enzyme stabilization and immobilization by sol-gel entrapment.- Nanoporous Gold for Enzyme Immobilization.- Enzyme stabilization via bio-templated silicification reactions.- The immobilization of enzyme on Eupergit supports by covalent attachment.- Micellar polymer encapsulation of enzymes.- Enzyme Stabilization via Cross-Linked Enzyme Aggregates.- Enzyme immobilization in polyelectrolyte microcapsules.- Macroporous poly(GMA-co-EGDMA) for enzyme stabilization.- Enzyme-nanoparticle conjugates for biomedical applications.- Enzyme nanoparticle fabrication- Magnetic nanoparticle synthesis and enzyme immobilization.- Cyhrome C stabilization and immobilization in aerogels.- Kinetic Measurement for Enzyme Immobilization.