Enzyme Activity in Single Cells

by Elsevier Science

Overview

Enzyme Activity in Single Cells, Volume 628, the latest release in the Methods of Enzymology series, discusses groundbreaking cellular physiology research that is taking place in the biological sciences. Chapters in this new release cover Spatial and temporal resolution of caspase waves in single Xenopus eggs during apoptosis, Spatial and temporal organization of metabolic complexes in cells, Measuring cellular efflux and biomolecular delivery: synthetic approaches to imaging and engineering cells, Slide-based, single-cell enzyme assays, Single-cell assays using integrated continuous-flow microfluidics, High-throughput screening of single-cell lysates, Microfluidic capture of single cells for drug resistance assays, and much more.

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in the Methods in Enzymology series
• Includes the latest information on Enzyme Activity in Single Cells

Product Details

ISBN-13:	9780128170915
Publisher:	Elsevier Science
Publication date:	10/31/2019
Series:	ISSN
Sold by:	Barnes & Noble
Format:	eBook
Pages:	326
File size:	46 MB
Note:	This product may take a few minutes to download.

About the Author

Nancy L. Allbritton is the Kenan Professor of Chemistry and Biomedical Engineering and Chair of the Joint Department of Biomedical Engineering at the University of North Carolina at Chapel Hill (UNC) and North Carolina State University (NC State). Her research focuses on the development of novel technologies for applications in single-cell analysis, micro-arrays and fluidics, and organ-on-chip. Dr. Allbritton is a Fellow of the American Association for the Advancement of Science, the American Institute for Medical&Biological Engineering, and the National Academy of Inventors. She obtained her B.S. in physics from Louisiana State University, M.D. from Johns Hopkins University, and Ph.D. in Medical Physics/Medical Engineering from the Massachusetts Institute of Technology, with a postdoctoral fellowship at Stanford University.
Michelle Kovarik is a bioanalytical chemist with particular interests in microfluidics, single-cell assays, and cellular responses to stress. She received her undergraduate degree from Saint Louis University and her doctoral degree from Indiana University. After a SPIRE postdoctoral appointment at the University of North Carolina, she began a tenure-track position at Trinity College, an undergraduate liberal arts college in Hartford, CT. In addition to her laboratory research, she also makes professional contributions in the area of chemistry education.

Table of Contents

1. Phase-separated condensates of metabolic complexes in living cells: Purinosome and glucosome Songon An, Miji Jeon, Erin L. Kennedy and Minjoung Kyoung 2. Synthetic probe development for measuring single or few-cell activity and efflux Alison Lui, Jeffrey Wang, Linda Chio and Markita P. Landry 3. Enzymatic activity in single cells Josephine Geertsen Keller, Magnus Stougaard and Birgitta R. Knudsen 4. Single-cell assays using integrated continuous-flow microfluidics Ee Xien Ng, Myat Noe Hsu, Guoyun Sun and Chia-Hung Chen 5. Single-cell activity screening in microfluidic droplets Stefanie Neun, Tomasz S. Kaminski and Florian Hollfelder 6. The microfluidic capture of single breast cancer cells for multi-drug resistance assays Karan Parekh, Hamide Sharifi, Avid Khamenehfar, Timothy V. Beischlag, Robert T.M. Payer and Paul C.H. Li 7. Single-cell proteolytic activity measurement using microfluidics for rare cell populations Yu-Chih Chen and Euisik Yoon 8. Microfluidic technology for investigation of protein function in single adherent cells Aldo Jesorka, Inga Põldsalu and Irep Gözen 9. Nanokits for the electrochemical quantification of enzyme activity in single living cells Rongrong Pan and Dechen Jiang 10. Design of an automated capillary electrophoresis platform for single-cell analysis David H. Abraham, Matthew M. Anttila, Luke A. Gallion, Brae V. Petersen, Angela Proctor and Nancy L. Allbritton 11. Microelectrophoretic single-cell measurements with microfluidic devices Jay Sibbitts, Jalal Sadeghi and Christopher T. Culbertson 12. Chemical probes for spatially resolved measurement of active enzymes in single cells Gang Li and Raymond E. Moellering 13. Single-cell proteomics in complex tissues using microprobe capillary electrophoresis mass spectrometry Camille Lombard-Banek, Sam B. Choi and Peter Nemes 14. Electroosmotic extraction coupled to mass spectrometry analysis of metabolites in live cells Ruichuan Yin, Venkateshkumar Prabhakaran and Julia Laskin