Embryonic Stem Cell Protocols: Volume II: Differentiation Models / Edition 2

by Kursad Turksen

Overview

Now in two volumes, this completely updated and expanded edition of Embryonic Stem Cells: Methods and Prools provides a diverse collection of readily reproducible cellular and molecular prools for the manipulation of nonhuman embryonic stem cells. Volume two, Embryonic Stem Cell Prools: Differentiation Models, Second Edition, covers state-of-the-art methods for deriving many types of differentiating cells from ES cells. The first volume, Embryonic Stem Cell Prools: Isolation and Characterization, Second Edition, provides a diverse collection of readily reproducible cellular and molecular prools for the isolation, maintenance, and characterization of embryonic stem cells. Together, the two volumes illuminate for both novices and experts our current understanding of the biology of embryonic stem cells and their utility in normal tissue homeostasis and regenerative medicine applications.

Product Details

ISBN-13:	9781617377778
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/19/2010
Series:	Methods in Molecular Biology , #330
Edition description:	Softcover reprint of hardcover 2nd ed. 2006
Pages:	456
Product dimensions:	5.98(w) x 9.02(h) x 0.04(d)

Table of Contents

Isolation and Maintenance.- Neural Stem Sphere Method.- Generation and Characterization of Oligodendrocytes From Lineage-Selectable Embryonic Stem Cells In Vitro.- Derivation and Characterization of Neural Cells From Embryonic Stem Cells Using Nestin Enhancer.- Optimized Neuronal Differentiation of Murine Embryonic Stem Cells.- Generation of Inner Ear Cell Types From Embryonic Stem Cells.- Derivation of Epidermal Colony-Forming Progenitors From Embryonic Stem Cell Cultures.- Directing Epidermal Fate Selection by a Novel Co-Culture System.- Signaling in Embryonic Stem Cell Differentiation.- In Vitro Generation of T Lymphocytes From Embryonic Stem Cells.- The Role of Hex in Hemangioblast and Hematopoietic Development.- Generation of Osteoblasts and Chondrocytes From Embryonic Stem Cells.- Analysis of Embryonic Stem Cell-Derived Osteogenic Cultures.- Generation of Chondrocytes From Embryonic Stem Cells.- Derivation and Characterization of Chondrocytes From Embryonic Stem Cells In Vitro.-Generation and Characterization of Cardiomyocytes Under Serum-Free Conditions.- Analysis of Arrhythmic Potential of Embryonic Stem Cell-Derived Cardiomyocytes.- Derivation and Characterization of Alveolar Epithelial Cells From Murine Embryonic Stem Cells In Vitro.- Genetic Manipulation of Embryonic Stem Cells.- Derivation and Characterization of Thyrocyte-Like Cells From Embryonic Stem Cells In Vitro.- Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells.- Formation of Gut-Like Structures In Vitro From Mouse Embryonic Stem Cells.- In Vitro Derivation and Expansion of Endothelial Cells From Embryonic Stem Cells.- Differentiation of Mouse Embryonic Stem Cells Into Endothelial Cells.- Integrins and Vascular Development in Differentiated Embryonic Stem Cells In Vitro.- TGF-β Signaling in Embryonic Stem Cell-Derived Endothelial Cells.- Use of Embryonic Stem Cells in Pharmacological and Toxicological Screens.- The Role of the Adapter Protein SHB in Embryonic StemCell Differentiation Into the Pancreatic—-Cell and Endothelial Lineages.- In Vitro Differentiation of Embryonic Stem Cells Into the Pancreatic Lineage.- Derivation and Characterization of Hepaytes From Embryonic Stem Cells In Vitro.- Differentiation of Embryonic Stem Cells to Retinal Cells In Vitro.- Epigenetic Analysis of Embryonic Stem Cells.- Derivation and Characterization of Lentoid Bodies and Retinal Pigment Epithelial Cells From Monkey Embryonic Stem Cells In Vitro.- Differentiation of Rhesus Monkey Embryonic Stem Cells in Three-Dimensional Collagen Matrix.

Editorial Reviews

From Reviews of the First Edition...

"...elegantly introduces tremendous methods and prools in ES studies...one of the most useful books that I have ever read in this field..." -Cell Biology International

"...highly valuable for any scientist who wants to make a start in the exciting field but also for experienced ES cell researchers who want to widen their repertoire" -Diabetologia

"...a very informative resource for any developmental or cell biologist with an interest in developments and prospects of ES cell research" -Molecular Biotechnology

"...a useful companion volume to other more specialized ES cell books..." -Nature Cell Biology

From the Publisher

Reviewer: Bruce A. Fenderson, PhD(Thomas Jefferson University)
Description: Stem cells are characterized by their ability to undergo self-renewal, while retaining the potential for terminal differentiation. There is hope that embryonic stem (ES) cells may someday provide tailor-made replacement cells and tissues for patients with chronic diseases (regenerative medicine). This is part of a two-volume set that describes laboratory protocols and experimental models that are currently used in stem cell research. These books are part of the popular Methods in Molecular Biology series. Volume 1 describes methods for stem cell isolation and characterization. Volume 2 provides examples of differentiation models. The two volumes include 62 chapters on topics ranging from isolation of Medaka ES cells and use of ES cells in embryotoxicity assays, to oligodendrocytes from lineage-selectable ES cells. The primary focus is on cell biology, biochemistry, and molecular biology.
Purpose: According to the editor, the aims of these books are to "complement the original mouse ES cell protocol book...focus on ES cells from non-mouse species...and provide new protocols for the use of ES cells in studies of diverse cell lineages." The editor hopes that these research-oriented protocols will help accelerate the pace of discovery in this field.
Audience: These stem cell protocol books are written for basic science and clinical researchers interested in cellular and developmental biology, biochemistry, and molecular biology. Biomedical researchers interested in bioengineering, gene therapy, oncology, and regenerative medicine will also appreciate themethods described here.
Features: Volume 1 is divided into seven major sections, including sections on signaling in ES cell differentiation, genetic manipulation of ES cells, and tumor-like properties of ES cells. Volume 2 includes chapters purporting to illustrate the conversion of nondescript ES cells to a wide variety of differentiated cell types, including lymphocytes, neurons, endothelial cells, and cardiomyocytes to name a few. Each chapter provides a summary, introduction, detailed step-by-step protocols, helpful notes, and references. The notes section allows the authors to provide rationales, further details, and tips for success. The figures and illustrations are reproduced in black and white. The authors are experts in this field.
Assessment: The major attraction of this two-volume set is the wide range of topics it covers. It is interesting to look at examples of how ES cells are derived from fish, chickens, and other species. An important issue raised in Volume 1 concerns the propensity of ES cells to form tumors in vivo. Clearly, there are major hurdles that must be cleared in order to make cell engineering safe and clinically effective. The protocols are typically organized around specific research questions, and numerous examples of research findings are presented. Thus, in addition to gaining access to methods, the reader benefits from a review of research questions under active investigation. One caveat is that protocols for lineage-directed differentiation need to be considered work in progress. This field is hampered by a lack of definitive markers for differentiated cells. Morphology is insufficient evidence for terminal differentiation and normal physiological function. These books will be a welcome resource for research scientists interested in stem cell biology.