Electrophoresis of Enzymes: Laboratory Methods
The electrophoresis of enzymes and isoenzymes is a well established technique in biochemical, clinical, environmental, microbiological, botanical and forensic laboratories and classical electrophoresis is presently undergoing a remarkable revival.
This book compiles facts and methods on enzyme electrophoresis widely dispersed in hundreds of publications. The author summarizes them in clearly readable tables, in many carefully worked out electrophoresis and more than 140 staining prools. The exhaustive practical experience of the author and the wealth of material summarized and reviewed makes this book a "must" for every enzyme laboratory. It will supply the practitioner with profound information on state-of-the-art enzyme electrophoresis.
"1117396140"
Electrophoresis of Enzymes: Laboratory Methods
The electrophoresis of enzymes and isoenzymes is a well established technique in biochemical, clinical, environmental, microbiological, botanical and forensic laboratories and classical electrophoresis is presently undergoing a remarkable revival.
This book compiles facts and methods on enzyme electrophoresis widely dispersed in hundreds of publications. The author summarizes them in clearly readable tables, in many carefully worked out electrophoresis and more than 140 staining prools. The exhaustive practical experience of the author and the wealth of material summarized and reviewed makes this book a "must" for every enzyme laboratory. It will supply the practitioner with profound information on state-of-the-art enzyme electrophoresis.
109.99 In Stock
Electrophoresis of Enzymes: Laboratory Methods

Electrophoresis of Enzymes: Laboratory Methods

by Gunter M. Rothe
Electrophoresis of Enzymes: Laboratory Methods

Electrophoresis of Enzymes: Laboratory Methods

by Gunter M. Rothe

Paperback(Softcover reprint of the original 1st ed. 1994)

$109.99 
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Overview

The electrophoresis of enzymes and isoenzymes is a well established technique in biochemical, clinical, environmental, microbiological, botanical and forensic laboratories and classical electrophoresis is presently undergoing a remarkable revival.
This book compiles facts and methods on enzyme electrophoresis widely dispersed in hundreds of publications. The author summarizes them in clearly readable tables, in many carefully worked out electrophoresis and more than 140 staining prools. The exhaustive practical experience of the author and the wealth of material summarized and reviewed makes this book a "must" for every enzyme laboratory. It will supply the practitioner with profound information on state-of-the-art enzyme electrophoresis.

Product Details

ISBN-13: 9783642790713
Publisher: Springer Berlin Heidelberg
Publication date: 12/15/2011
Series: Springer Lab Manuals
Edition description: Softcover reprint of the original 1st ed. 1994
Pages: 307
Product dimensions: 6.69(w) x 9.53(h) x 0.03(d)

Table of Contents

1 Introduction.- 1.1 Enzyme Classification.- 1.2 Enzyme Subunit Composition.- 1.3 Isozyme Classification.- 1.4 References.- 2 Extraction of Enzymes from Tissues, Cells and Cell-Organelles.- 2.1 Methods to Extract Enzymes from Microorganisms.- 2.2 Methods to Extract Enzymes from Animal Soft Tissue.- 2.3 Differential Extraction of Cytosolic and Mihondrial Enzymes from Animal Soft Tissue.- 2.4 Extraction of Insects.- 2.5 Extraction of Plant Tissues.- 2.6 Concentration of Diluted Enzyme Extracts.- 2.7 Mammalian Blood.- 2.8 Pancreatic Islets.- 2.9 Isolation of Subcellular Organelles.- 2.10 Catalytic Activities of Enzymes Forming a Common Metabolic Sequence.- 2.11 References.- 3 Methods for Separating Native Enzymes.- 3.1 General Considerations.- 3.2 Cellulose Acetate Electrophoresis.- 3.3 Starch Gel Electrophoresis.- 3.4 References.- 3.5 Analytical Polyacrylamide Gel Electrophoresis for Separating Native Enzymes.- 3.6 2-D-Electrophoresis under Non-Denaturing Conditions.- 3.7 References.- 4 Sodium Dodecylsulphate Electrophoresis.- 4.1 Homogeneous Buffer Systems.- 4.2 Discontinuous Buffer Systems.- 4.3 Gradient Gel Systems.- 4.4 Enzyme Visualization Following SDS-Electrophoresis.- 4.5 References.- 5 Chemistry of Enzyme Visualization.- 5.1 Methods for Visualizing Oxidative Enzymes.- 5.2 Methods for Visualizing Transferases.- 5.3 Methods for Visualizing Hydrolases.- 5.4 Methods for Visualizing Lyases.- 5.5 Methods for Localizing Isomerases and Ligases.- 5.6 Visualization of Enzymes by Different Methods.- 5.7 References.- 6 A Compilation of Prools to Visualize Enzymes.- 6.1 Index to Enzyme Recipes and Separation Systems.- 6.2 Prools to Visualize Enzymes Following Electrophoretic Separation.- 6.3 References.- 7 Data Evaluation in Population Genetics and Evolution.- 7.1 Allozymes as Gene Markers.- 7.2 Population Genetic Measures.- 7.3 Calculation of Dendrograms.- 7.4 References.
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