DNA Repair Protocols / Edition 1

by Daryl S. Henderson

Overview

The first edition of this laboratory manual was published in 1999 under the title DNA Repair Protocols: Eukaryotic Systems. In the second edition, the focus has been narrowed to allow more in-depth coverage of mammalian cells in particular. The 32 contributions from international researchers describe a wide range of easily reproducible techniques for the study of DNA repair. Each chapter uses a common format that includes step-by-step instructions, lists of necessary equipments and reagents, and troubleshooting tips. Editor Henderson teaches pharmacological sciences at the State U. of New York, Stony Brook. Annotation © 2006 Book News, Inc., Portland, OR

Product Details

ISBN-13:	9781588295132
Publisher:	Springer-Verlag New York, LLC
Publication date:	10/01/2005
Series:	Methods in Molecular Biology , #314
Edition description:	2006
Pages:	498
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating.- Complementation Assays Adapted for DNA Repair-Deficient Keratinocytes.- Cytogenetic Challenge Assays for Assessment of DNA Repair Capacities.- Evaluating the Delayed Effects of Cellular Exposure to Ionizing Radiation.- Inhibition of DNA Synthesis by Ionizing Radiation.- Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication.- Cytometric Assessment of Histone H2AX Phosphorylation.- Detection of DNA Strand Breaks by Flow and Laser Scanning Cytometry in Studies of Apoptosis and Cell Proliferation (DNA Replication).- In Vitro Rejoining of Double-Strand Breaks in Genomic DNA.- Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR.- Plasmid-Based Assays for DNA End-Joining In Vitro.- Use of Gene Targeting to Study Recombination in Mammalian Cell DNA Repair Mutants.- Gene-Specific and Mihondrial Repair of Oxidative DNA Damage.- Quantitative PCR-Based Measurement of Nuclear and Mihondrial DNA Damage and Repair in Mammalian Cells.- Measuring the Formation and Repair of DNA Damage by Ligation-Mediated PCR.- Immunochemical Detection of UV-Induced DNA Damage and Repair.- A Dot-Blot Immunoassay for Measuring Repair of Ultraviolet Photoproducts.- Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay.- DNA Damage Quantitation by Alkaline Gel Electrophoresis.- The Comet Assay.- Fast Micromethod DNA Single-Strand-Break Assay.- 32P-Postlabeling DNA Damage Assays.- Electrophoretic Mobility Shift Assays to Study Protein Binding to Damaged DNA.- Construction of MMR Plasmid Substrates and Analysis of MMR Error Correction and Excision.- Characterization of Enzymes ThatInitiate Base Excision Repair at Abasic Sites.- Base Excision Repair in Mammalian Cells.- In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts.- Biochemical Assays for the Characterization of DNA Helicases.- Repair Synthesis Assay for Nucleotide Excision Repair Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA.- Assaying for the Dual Incisions of Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site.- Analysis of Proliferating Cell Nuclear Antigen (PCNA) Associated With DNA Excision Repair Sites in Mammalian Cells.- Analysis of DNA Repair and Chromatin Assembly In Vitro Using Immobilized Damaged DNA Substrates.

Editorial Reviews

"...It will serve as an essential reference for both the practical and theoretical aspects of DNA repair studies, encourage the transfer of methodologies between model systems, and stimulate the development of new approaches." - Anticancer Research

"...must for any science library supporting a molecular biology program." - E-Streams

"An informative collection of techniques and prools for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in DNA repair field. This collection of prools is ideal for both the novice in the field and to the experienced applied researcher." - Lavoisier Librairie

"...an extremely useful resource for any laboratory with an interest in the vibrant field of DNA repair. Any good laboratory manueal should be not only a useful reference guide but also a source of inspiration, and it is undoubtedly true that DNA Repair Prools: Mammalian Systems succeeds on both counts." -Int. J. Radiation Oncology Biol. Phys.

"This is the definite book on the subject not only for those working in the field of DNA repair but also for those interested in genetic toxicology and chemical safety." -BTS Newsletter

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