DNA Cloning: A Practical ApproachVolume 2: Expression Systems / Edition 2

by D. M. Glover, B. D. Hames

Overview

Expression systems are in widespread use for cloning specific genes, for synthesizing the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories.

Product Details

ISBN-13:	9780199634781
Publisher:	Oxford University Press
Publication date:	06/22/1995
Series:	Practical Approach Series , #149
Edition description:	REV
Pages:	274
Product dimensions:	9.18(w) x 6.38(h) x 0.65(d)

About the Author

Glover, D. M. (Univ of Dundee); Hames, B. D. (Univ of Leeds)

The contributors represent the specialties of molecular biology, cell biology, and biochemistry. Most are from hospitals, academic medical centers, and laboratories in the U.S. and the U.K. Institutions prominently represented include Massachusetts General Hospital, Univ of Wisconsin at Madison, Wellcome Research Laboratories, and Univ of Dundee.

Table of Contents

1. Screening expression libraries with antibody and protein probes2. Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies3. Purification of over-produced proteins from E.coli cells4. Production of monoclonal antibodies against proteins expressed in E.coli5. Expression of cloned genes in yeast6. Intraction trap cloning with yeast7. The baculovirus expression system

Editorial Reviews

3 Stars from Doody

This second edition incorporates contributions from established researchers in the field. It covers a wide range of current laboratory procedures for the expression of cDNA clones in a variety of expression systems together with the methods for screening libraries, purification of desired proteins, and the generation of monoclonal and polyclonal antibodies. The purpose is to provide theoretical and practical guidance in the basic methods needed for controlled production of gene products. The book largely meets its overall objective. Although intended especially to guide newcomers into studies involving expression of cloned genes, this book will also prove useful for experienced researchers. The contributors are active research workers who have important contributions to their respective areas, and so have first-hand knowledge of the topics they address. This nicely produced book is an excellent collection of established protocols in a step by step, ^^cookbook^^ format. Each chapter begins with a brief introduction giving an overview of the methods, followed by a complete list of equipment and reagents required and an up-to-date protocol. Each chapter concludes with useful hints, tips, and troubleshooting comments together with a concise list of references for additional information. This is an extremely useful book for anyone interested in learning and mastering the techniques currently used for controlled production of gene products. One problem is that the book is bound in a conventional fashion, as compared to other manuals that come as a spiral notebook. Comb binding will facilitate its use on a laboratory bench, its natural habitat. Nevertheless, the book helps fill the need for acomprehensive manual designed especially for the novice.

Arshad Rahman

Reviewer: Arshad Rahman, PhD (Rush Medical College of Rush University)
Description: This second edition incorporates contributions from established researchers in the field. It covers a wide range of current laboratory procedures for the expression of cDNA clones in a variety of expression systems together with the methods for screening libraries, purification of desired proteins, and the generation of monoclonal and polyclonal antibodies.
Purpose: The purpose is to provide theoretical and practical guidance in the basic methods needed for controlled production of gene products. The book largely meets its overall objective.
Audience: Although intended especially to guide newcomers into studies involving expression of cloned genes, this book will also prove useful for experienced researchers. The contributors are active research workers who have important contributions to their respective areas, and so have first-hand knowledge of the topics they address.
Features: This nicely produced book is an excellent collection of established protocols in a step by step, "cookbook" format. Each chapter begins with a brief introduction giving an overview of the methods, followed by a complete list of equipment and reagents required and an up-to-date protocol. Each chapter concludes with useful hints, tips, and troubleshooting comments together with a concise list of references for additional information.
Assessment: This is an extremely useful book for anyone interested in learning and mastering the techniques currently used for controlled production of gene products. One problem is that the book is bound in a conventional fashion, as compared to other manuals that come as a spiral notebook. Comb binding will facilitate its use on a laboratory bench, its natural habitat. Nevertheless, the book helps fill the need for a comprehensive manual designed especially for the novice.

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