Cytochemical Staining Methods for Electron Microscopy

by P.R. Lewis, D.P. Knight

Overview

The series Practical Methods in Electron Microscopy, edited by Audrey M. Glauert has an international reputation as a unique source of practical information for all electron microscopists.
Each book of the series starts from first principles, assuming no specialist knowledge, and is complete in itself. The series will eventually cover the whole range of techniques for electron microscopy.

In this latest volume in the popular series, Peter Lewis and David Knight describe in practical detail the whole range of staining techniques for electron microscopy, including contrast staining for ultrastructural studies, specific cytological staining methods, and enzyme cytochemical techniques.

The chemical basis of cytochemical methods is discussed and analysed in a straightforward manner with great clarity. In consequence this book will help the beginner to choose between the sometimes bewildering variety of published technical procedures.

Electron microscopists will find this volume to be an invaluable and compact source-book for all those cytochemical techniques that have proved to be both reliable and convenient to use. As with previous titles in this popular series, the unique type of practical guidance provided will be of value for many years to come.

Product Details

ISBN-13:	9780444893871
Publisher:	Elsevier Science
Publication date:	08/04/1992
Series:	Practical Methods in Electron Microscopy , #14
Pages:	340
Product dimensions:	0.71(w) x 6.14(h) x 9.21(d)

Table of Contents

Chapter 1. Introduction (P.R. Lewis and D.P. Knight). 1.1. The Objectives of
Section Staining. 1.2. Contrast in Transmission Electron Microscopy. 1.3.
Fixation of tissues. 1.4. Preparation of Tissues. 1.5. Special Embedding
Techniques. 1.6. Safety in the Laboratory. Chapter 2. Cytological Staining
Methods (D.P. Knight and P.R. Lewis). 2.1. Resin Sections for Light
Microscopy. 2.2. Procedures for Handling Ultrathin Sections. 2.3. Lead
Stains. 2.4. Uranyl Acetate. 2.5. A Procedure for Double Staining. 2.6.
Osmium Tetroxide. 2.7. Other Cytological Stains. 2.8. Tracers in Electron
Microscopy. Chapter 3. General Cytochemical Methods (D.P. Knight and P.R.
Lewis). 3.1. The limitations of present techniques. 3.2. Cytochemical
Methods with Broad Specificities. 3.3. Methods for Nucleic Acids. 3.4.
General Methods for Proteins. 3.5. Specific Methods for Carbohydrates. 3.6.
Methods for Lipids. 3.7. Precipitation Methods for Inorganic Ions. 3.8.
Extractive Methods. Chapter 4. Metal Precipitation Methods for Hydrolytic
Enzymes (P.R. Lewis). 4.1. The Principles of Enzyme Cytochemistry. 4.2.
Some Practical Problems of Enzymes Cytochemistry. 4.3. Methods for Acid
Hydrolases and Related Techniques. 4.4. Enzymes Which Hydrolyze ATP. 4.5.
Other Phosphatases. 4.6. General Techniques for Esterases. 4.7. Techniques
Specific for the Cholinesterases. Chapter 5. Other Cytochemical Methods for
Enzymes (P.R. Lewis). 5.1. Histochemistry of the Oxidases. 5.2. A
Biochemical Background to Dehydrogenase Histochemistry. 5.3. Ferricyanide as a Histochemical Reagent. 5.4. The Use of Tetrazolium Salts. 5.5. Azo-dye Coupling Techniques. 5.6 Methods for Other Enzymes. 5.7. Recent
Advances and Future Development in Cytochemistry. Appendix 1. Stock solutions. Appendix 2. List of suppliers.

Editorial Reviews

"...another outstanding volume in...truly informative and interesting examples...explicitly written chapters are well illustrated...highly recommended as an essential reference work for any EM laboratory." —Scanning

"When the first edition was published in 1977 it became essential reading, to new and existing users; this updated second editionis equally essential." —Microscopy and Analysis

From the Publisher