CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases

by Luis María Vaschetto (Editor)

Overview

The CRISPR-Cas9 genome-editing system is creating a revolution in the science world. In the laboratory, CRISPR-Cas9 can efficiently be used to target specific genes, correct mutations and regulate gene expression of a wide array of cells and organisms, including human cells. CRISPR-/Cas9 Based Genome Editing for Treating Genetic Disorders and Diseases is a unique reading material for college students, academicians, and other health professionals interested in learning about the broad range of applications of CRISPR/Cas9 genetic scissors. Some topics included in this book are: the role of the CRISPR/Cas9 system in neuroscience, gene therapy, epigenome editing, genome mapping, cancer, virus infection control strategies, regulatory challenges and bioethical considerations.

Product Details

ISBN-13:	9781000540833
Publisher:	CRC Press
Publication date:	01/31/2022
Sold by:	Barnes & Noble
Format:	eBook
Pages:	268
File size:	6 MB

About the Author

Luis María Vaschetto received his Doctorate in Biological Sciences from Universidad Nacional de Córdoba (Argentina). He was a postdoctoral researcher at the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and a research associate at South Dakota State University (SDSU). His research focuses on understanding the epigenetic mechanisms involved in plant and animal development, as well as their effects on human health and diseases.

Table of Contents

1. The CRISPR/Cas9 Genome-editing System: Principles and Applications 2. Recent Applications of CRISPR-Cas9 in Genome Mapping and Sequencing 3. Gene Editing and Genetic Disorders: Ethical and Legal Concerns 4. Political, Regulatory and Ethical Considerations of the CRISPR/Cas Genome Editing Technology 5. CRISPR/Cas and Gene Therapy: An Overview 6. CRISPR and Cancer: From Bench to Clinic 7. The Advances of the CRISPR/Cas9 Technology for Correcting Genetic Disorders: Using Sarcoma as a Model System 8. CRISPR/Cas9 Technology as a Strategy Against Viral Infections 9. CRISPR/Cas9-based Genome and Epigenome Editing in Neuroscience Research 10. Non-viral Alternatives to Delivery of CRISPR-Cas Gene Editing Components 11. CRISPR Medicine: Advance, Progress and Challenges

Editorial Reviews

Reviewer: Mark F. Sanders, PhD (UC Davis College of Biological Sciences)
Description: The CRISPR/CAS9 genome editing system and its application to the treatment of human diseases and genetic disorders is the topic of this volume. The book provides background information on CRISPR/CAS9, discussions of the bioethics of the use of the system in humans, descriptions of numerous applications to date, and discussion of limitations and problems using the method for human genome editing.
Purpose: The editor and authors of this volume intend it as an update and summary of the CRISPR/CAS9 in humans. Through the targeting of specific genes, genome editing is seen to have wide-ranging potential for improvements in plant and animal agriculture and for the treatment of diseases and inherited disorders in humans. This book limits itself largely to discussions of applications in humans and, in doing so, it contributes a much-needed summary of successes and limitations of applications to date.
Audience: There is a wide potential audience for this book. Medical researchers and medical practitioners who are actively engaged in research in the field of genome editing will find the book to be a concise summary of much of the important work to date. Similarly, those interested in learning more about the potential applications of CRISPR/CAS9 will find the largely self-encapsulated chapters highly informative. A diverse group of more than 30 authors with strong expertise in their respective areas of focus have been assembled to produce this excellent volume.
Features: This is a concise volume of approximately 260 pages that is divided into 11 chapters that are collectively the work of more than 30 authors. Nearly every chapter can be treated as a stand-alone chapter in that each provides an appropriate summary of CRISPR/CAS9 as it relates to the topic of the chapter and gives an appropriate description of the application and outcomes of genome editing. The book begins with a well written overview of CRISPR/CAS9. This is followed by two chapters on the bioethics of human genome editing. The eight follow-on technical chapters describe advances in the application of genome editing to inherited disorders and to the treatment of cancer and other diseases. A chapter on delivery systems for CRISPR/CAS9 follows and an effective summary sets future directions for the field. The illustrations in the book are of variable quality and subpar in comparison to the quality that could have been achieved with more careful editorial work.
Assessment: Overall, this is an excellent book and a valuable contribution to the burgeoning field of human genome editing. Each chapter is well sourced and has an extensive list of references. Indeed, more than 25 percent of the 259 numbered pages in the book are devoted to references. This book offers an excellent starting point for exploration of genome editing in humans and is highly recommended.

Doody's Review Service

'Overall, this is an excellent book and a valuable contribution to the burgeoning field of human genome editing. Each chapter is well sourced and has an extensive list of references. Indeed, more than 25 percent of the 259 numbered
pages in the book are devoted to references. This book offers an excellent starting point for exploration of genome editing in humans and is highly recommended.'

- Mark F. Sanders, PhD, UC Davis College of Biological Sciences

From the Publisher