Contemporary Enzyme Kinetics and Mechanism: Selected Methods in Enzymology

by Daniel L. Purich (Editor)

Overview

Selected Methods in Enzymology: Contemporary Enzyme Kinetics and Mechanism provides an introduction to enzyme kinetics and mechanism at an intermediate level. This book covers a variety of topics, including temperature effects in enzyme kinetics, cryoenzymology, substrate inhibition, enol intermediates enzymology, and heavy-atom isotope effects. Organized into 19 chapters, this book begins with an overview of derivation of rate equations as an integral part of the effective usage of kinetics as a tool. This text then examines the practical aspects of initial rate enzyme assay. Other chapters consider the basic procedures used in making decisions concerning kinetic mechanisms from initial-rate data. This book discusses as well the various aspects of both the theoretical background and the applications. The final chapter deals with the importance of achieving proficiency in formulating quantitative relationships describing enzyme behavior. This book is a valuable resource for students and research workers. Enzymologists and chemists will also find this book useful.

Product Details

ISBN-13:	9781483214221
Publisher:	Elsevier Science
Publication date:	01/01/1983
Sold by:	Barnes & Noble
Format:	eBook
Pages:	566
File size:	9 MB

About the Author

Daniel Lee Purich has been at the forefront of biochemistry research for more than 25 years. He was awarded the National Institutes of Health Research Career Development Award from 1977–1982, the Plous Teaching Award (the University of California Santa Barbara Campus-Wide Teaching Award) in 1977, has been a member of the National Institutes of Health Biochemistry Study Section from 1982–1985, and a member of the Journal of Biological Chemistry Editorial Board from 1981–1986. He has been a member of the American Society of Biological Chemists, the American Chemical Society, the New York Academy of Sciences, the Biochemical Society, and the American Society for Cell Biology. Dr. Purich is currently a Professor and Chairman of the Department of Biochemistry and Molecular Biology at the Florida College of Medicine. He is the author and editor of numerous scientific publications.

Table of Contents

List of Contributors

Foreword

Preface

Contents of Methods In Enzymology Volumes 63, 64, and 87

1. Derivation of Initial Velocity and Isotope Exchange Rate Equations

2. Practical Considerations in the Design of Initial Velocity Enzyme Rate Assays

3. Plotting Methods for Analyzing Enzyme Rate Data

4. Regression Analysis, Experimental Error, and Statistical Criteria in the Design and Analysis of Experiments for Discrimination Between Rival Kinetic Models

5. Effects of pH on Enzymes

6. Temperature Effects in Enzyme Kinetics

7. Cryoenzymology: The Study of Enzyme Catalysis at Subzero Temperatures

8. Product Inhibition and Abortive Complex Formation

9. Use of Competitive Inhibitors to Study Substrate Binding Order

10. Substrate Inhibition

11. Cooperativity in Enzyme Function: Equilibrium and Kinetic Aspects

12. Application of Affinity Labeling for Studying Structure and Function of Enzymes

13. Criteria for Evaluating the Catalytic Competence of Enzyme-Substrate Covalent Compounds

14. Enzymology of Enol Intermediates

15. Stereochemistry of Enzymic Phosphoryl and Nucleotidyl Transfer

16. Isotope Exchange Methods for Elucidating Enzymic Catalysis

17. The Use of Isotope Effects to Determine Transition-State Structure for Enzyme Reactions

18. Determination of Heavy-Atom Isotope Effects on Enzyme-Catalyzed Reactions

19. Selected Exercises and Problems

Index

Editorial Reviews

3 Stars from Doody

This multiauthored second edition monograph is, as was the first edition published in 1983, a compilation of basic articles from the Enzymes and Kinetics series of Methods in Enzymology. The chapters were written by 19 contributors from various specialties. Most of the chapters have been updated, and 10 of the 17 chapters are new. "This monograph is meant to update the earlier volume to continue to provide assistance in design, execution and analysis of enzyme systems. Since the first edition in 1983, the dramatic growth of this area has necessitated a newer edition. For example, this edition has been updated extensively and, for example, now includes a very interesting chapter on site-directed mutagenesis. "Although the audience is meant to be biochemists and biophysicists, this monograph is an ideal primary textbook for students in this area. "This softcover book features the typical attractive Methods in Enzymology format, with a suitable number of black-and-white illustrations. The book also features a chapter on selected exercises and problems of both a quantitative and descriptive nature. "This book is highly recommended because it contains basic information and discussion that covers earlier work to more recent work on enzyme dynamics. It is also very important because of the increasing need for more nonspecialists to be able to study newly discovered or expressed enzymes.

Gene A. Homandberg

A collection of articles from vols. 63, 64, 87, and 249 of Methods in Enzymology, describing widely used strategies in kinetic studies of enzyme action and offering exercises and problems. Subjects include cooperativity in enzyme function, product inhibition applications, mechanism-based enzyme inactivators, and hydrogen tunneling in enzyme catalysis. Includes the complete table of contents for each of the volumes used in the collection. This second edition contains articles from vol. 249, and ten of the 17 chapters are new. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

Reviewer: Gene A. Homandberg, PhD (Rush Medical College of Rush University)
Description: This multiauthored second edition monograph is, as was the first edition published in 1983, a compilation of basic articles from the Enzymes and Kinetics series of Methods in Enzymology. The chapters were written by 19 contributors from various specialties. Most of the chapters have been updated, and 10 of the 17 chapters are new.
Purpose: This monograph is meant to update the earlier volume to continue to provide assistance in design, execution and analysis of enzyme systems. Since the first edition in 1983, the dramatic growth of this area has necessitated a newer edition. For example, this edition has been updated extensively and, for example, now includes a very interesting chapter on site-directed mutagenesis.
Audience: Although the audience is meant to be biochemists and biophysicists, this monograph is an ideal primary textbook for students in this area.
Features: This softcover book features the typical attractive Methods in Enzymology format, with a suitable number of black-and-white illustrations. The book also features a chapter on selected exercises and problems of both a quantitative and descriptive nature.
Assessment: This book is highly recommended because it contains basic information and discussion that covers earlier work to more recent work on enzyme dynamics. It is also very important because of the increasing need for more nonspecialists to be able to study newly discovered or expressed enzymes.

Doody's Review Service