Chemosensitivity: Volume I: In Vitro Assays / Edition 1

by Rosalyn D. Blumenthal

Overview

A state-of-the art collection of readily reproducible laboratory methods for assessing chemosensitivity in vitro and in vivo, and for assessing the parameters that modulate chemosensitivity in individual tumors. Chemosensitivity, Volume 1: In Vitro Assays provides a panel of 16 in vitro measures of chemosensitivity in adherent and non-adherent cells for single agents and combinations of agents. In addition to immunohishemical and imaging approaches, these assays include clonogenic, colorimetric, fluorometric, and physiological assays. Highlights include image analysis to assess drug sensitivity, high throughput approaches using green fluorescent protein, DIMSCAN (a microcomputer fluorescence-based assay), and the ChemoFx assay used in biotechnology. A companion volume, Volume 2: In Vivo Models, Imaging, and Molecular Regulators, provides prools for classifying tumors into response categories and customizing chemotherapy regimens to individual patients.

Product Details

ISBN-13:	9781588293459
Publisher:	Springer-Verlag New York, LLC
Publication date:	02/14/2005
Series:	Methods in Molecular Medicine , #110
Edition description:	2005
Pages:	231
Product dimensions:	6.10(w) x 9.25(h) x 0.02(d)

Table of Contents

An Overview of Chemosensitivity Testing.- Clonogenic Cell Survival Assay.- High-Sensitivity Cytotoxicity Assays for Nonadherent Cells.- Sulforhodamine B Assay and Chemosensitivity.- Use of the Differential Staining Cytotoxicity Assay to Predict Chemosensitivity.- Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity.- The MTT Assay to Evaluate Chemosensitivity.- Hisulture Drug Response Assay to Monitor Chemoresponse.- In Vitro Testing of Chemosensitivity in Physiological Hypoxia.- Chemosensitivity Testing Using Microplate Adenosine Triphosphate-Based Luminescence Measurements.- High-Throughput Technology.- Dimscan.- The ChemoFx® Assay.- Evaluating Response to Antineoplastic Drug Combinations in Tissue Culture Models.- Image Analysis Using the Fluorochromasia Assay to Quantify Tumor Drug Sensitivity.- Immunohishemical Detection of Ornithine Decarboxylase as a Measure of Chemosensitivity Testing.- Immunohishemistry of p53, Bcl-2, and Ki-67 as Predictors of Chemosensitivity.