This laboratory guide, intended for undergraduate and postgraduate students, includes techniques and their protocols ranging from microscopy to in vitro protein synthesis. Experiments relating to chromosomes study and identifying the phases of cell division are explained. The book lucidly deals with the extraction and characteri-zation of chromatin and techniques for studying its modifications, the gene methodology for identification of mutation and the methodology for isolation of nucleic acids from all types of organisms, such as viruses, fungi, plants and animals. All the protocols have been explained following step-by-step method. Different types of electrophoresis and their techniques, including blotting techniques and the methodology for stripping of probes from membranes for reusing the blot, have also been dealt with. Protocols on modern molecular biology techniques—PCR, restriction enzyme digest, DNA isolation, cloning and DNA sequencing—add weightage to the book. It also gives necessary knowledge of different types of stains, staining techniques, buffers, reagents and media used in the protocols.
To help students prepare for answering viva voce questions, the book includes MCQs based on the discussed techniques.
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About the Author
<strong>K. V. Chaitanya</strong>, PhD, is Associate Professor in the Department of Biotechnology at GITAM University, Visakhapatnam. He has a research experience of more than a decade and nearly five years of teaching experience. He worked on “response of mulberry plants to abiotic stress” for his M.Phil and doctoral degrees. He also worked on the “role of TCP genes” in the natural variation of lettuce leaf shape for four and half years in the Department of Microbiology and Cell Biology at Indian Institute of Science as DBT Postdoctoral Fellow for post-doctoral research. Dr. Chaitanya has published more than 20 articles in peer-reviewed international journals and three articles at the national level. He has also contributed articles to edited volumes.
Table of Contents
1. Microscopy<br /> 2. Cell Division<br /> 3. Chromosomes<br /> 4. Chromatin and Histone Proteins<br /> 5. Mutations<br /> 6. Isolation of Nucleic Acids<br /> 7. Estimation of DNA, RNA and Proteins<br /> 8. Electrophoresis<br /> 9. Blotting Techniques<br /> 10. Polymerase Chain Reaction<br /> 11. Restriction Digestion<br /> 12. Gene Cloning <br /> 13. DNA Sequencing<br /> 14. Protein Synthesis
Appendix A Preparation of Buffers and Reagents<br /> Appendix B General Laboratory Practices<br /> Appendix C Staining and Common Stains in Cytology<br /> Index