C. elegans: Methods and Applications / Edition 2

by David Biron, Gal Haspel

Overview

The aim of this volume is to provide a step-by-step guide for implementing a selection of novel techniques in the lab. Each prool in this volume is presented as a standalone chapter, specifically geared towards addressing practical needs without presuming prior knowledge of the technique at hand. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, C. elegans: Methods and Applications, Second Edition aims to ensure successful results in the further study of this dynamic field.

Product Details

ISBN-13:	9781493948925
Publisher:	Springer New York
Publication date:	08/23/2016
Series:	Methods in Molecular Biology , #1327
Edition description:	Softcover reprint of the original 2nd ed. 2015
Pages:	252
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Library Construction for Mutation Identification by Whole-Genome Sequencing.- Fundamentals of Comparative Genome Analysis in Caenorhabditis Nematodes.- Genetic Methods for Cellular Manipulations in C. elegans.- A Fusion PCR Method for Expressing Genetic Tools in C. elegans.- Transposon Assisted Genetic Engineering with Mos1 Mediated Single-copy Insertion (MosSCI).- Creating Genome Modifications in C. elegans using the CRISPR/Cas9 System.- Observing and Quantifying Fluorescent Reporters.- Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior.- Simultaneous Optogenetic Stimulation of Individual Pharyngeal Neurons and Monitoring of Feeding Behavior in Intact C. elegans.- High-Pressure Freeze and Freeze Substitution Electron Microscopy in C. elegans.- Electron Tomography Methods for C. elegans.- Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans.- Tracking Single C. elegans using a USB Microscope on a Motorized Stage.- An Imaging System for C. elegans Behavior.- A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans dauer Larvae.- Sampling and Isolation of C. elegans from the Natural Habitat.- A Primer on Prototyping.- A Primer on Quantitative Modeling.