Biotechnology Proteins to PCR: A Course in Strategies and Lab Techniques / Edition 1

by David W. Burden, Donald B. Whitney

Overview

This is a manual which aims to give students a chance to explore the process and techniques of characterizing and purifying a protein, and of subsequent cloning of the associated gene. Each chapter emphasizes the process of discovery, and the strategies and rationale for each experiment. To minimize cost and avoid extensive laboratory preparation, the experiments in this manual use readily available materials and organisms, the bacterium Escherichia coli and the yeast Saccharomyces carlsbergensis.

Product Details

ISBN-13:	9780817638436
Publisher:	Birkhäuser Boston
Publication date:	11/01/1995
Edition description:	1995
Pages:	317
Product dimensions:	6.69(w) x 9.61(h) x 0.03(d)

Table of Contents

1. Introduction to the Biotechnology Laboratory.- 1.1Overview.- 1.2 Background.- 1.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 2. Introduction to Proteins.- 2.1 Overview.- 2.2 Background.- 2.3 Experimental Design and Procedures.- Biuret Protein Assay.- Lowry Protein Assay.- Study Questions.- Further Readings.- 3. Protein Isolation and Preparation of Crude Extract.- 3.1 Overview.- 3.2 Background.- 3.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 4. Batch Purification of Proteins.- 4.1 Overview.- 4.2 Background.- 4.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 5. Protein Purification by Column Chromatography.- 5.1 Overview.- 5.2 Background.- 5.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 6. Protein Analysis and Verification.- 6.1 Overview.- 6.2 Background.- 6.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 7. Designing a Cloning Scheme.- 7.1 Overview.- 7.2 Background.- 73 Experimental Design and Procedures.- 8. Isolation and Preparation of Nucleic Acids.- 8.1 Overview.- 8.2 Background.- 8.3 Expermental Design and Procedures.- Study Questions.- Further Readings.- 9. Constructing a Gene Bank.- 9.1 Overview.- 9.2 Background.- 9.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 10. Introducing Recombinant Molecules into Escherichia coli.- 10.1 Overview.- 10.2 Background.- 10.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 11. Screening for Clones.- 11.1 Overview.- 11.2 Background.- 11.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 12. Characterizing and Verifying Cloned DNA.- 12.1 Overview.- 12.2 Background.- 12.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 13. Application of DNA Sequence and Clone Data.- 13.1 Overview.- 13.2 Background.- 13.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 14. The Value of Information.

Editorial Reviews

A textbook for an undergraduate or professional training course for students with a solid background in general biology and chemistry, and hopefully some organic chemistry; does not assume any microbiology and biochemistry. Explains the laboratory techniques and skills for characterizing and purifying a protein and for cloning the associated gene, and the strategies and rationale for the research process itself. The examples use readily available materials and organisms to keep the cost down. Annotation c. Book News, Inc., Portland, OR (booknews.com)

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