Biosensors and Biodetection: Methods and Protocols Volume 1: Optical-Based Detectors / Edition 1

by Avraham Rasooly, Keith Herold

Overview

Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of use compared to traditional detection methods. In Biosensors and Biodetection: Methods and Prools, leading experts describe the major technologies in the field in extensive technical detail, allowing readers both to understand the technology and to construct similar devices. Volume 1: Optical-Based Detectors delves into direct and indirect optical detectors, including methods involving surface plasmon resonance, interferometric sensors, CCD based detectors, and spectrometers, among many other cutting-edge technologies. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the subjects, lists of the necessary materials, step-by-step, readily reproducible prools, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Prools is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field.

Product Details

ISBN-13:	9781617379116
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/19/2010
Series:	Methods in Molecular Biology , #503
Edition description:	Softcover reprint of hardcover 1st ed. 2009
Pages:	452
Product dimensions:	0.00(w) x 0.00(h) x 0.04(d)

Table of Contents

Optical-Based Detectors.- Surface Plasmon Resonance and Surface Plasmon Field-Enhanced Fluorescence Spectroscopy for Sensitive Detection of Tumor Markers.- Surface Plasmon Resonance Biosensor for Biomolecular Interaction Analysis Based on Spatial Modulation Phase Detection.- Array-Based Spectral SPR Biosensor: Analysis of Mumps Virus Infection.- Optical Biosensors Based on Photonic Crystal Surface Waves.- Surface Plasmon Resonance Biosensing.- Label-Free Detection with the Resonant Mirror Biosensor.- Label-Free Detection with the Liquid Core Optical Ring Resonator Sensing Platform.- Reflectometric Interference Spectroscopy.- Phase Sensitive Interferometry for Biosensing Applications.- Label-Free Serodiagnosis on a Grating Coupler.- Indirect Detectors.- CCD Camera Detection of HIV Infection.- Simple Luminescence Detector for Capillary Electrophoresis.- Optical System Design for Biosensors Based on CCD Detection.- A Simple Portable Electroluminescence Illumination-Based CCD Detector.- Fluoroimmunoassays Using the NRL Array Biosensor.- Biosensors Technologies: Acousto-Optic Tunable Filter-Based Hyperspectral and Polarization Imagers for Fluorescence and Spectroscopic Imaging.- Photodiode-Based Detection System for Biosensors.- Photodiode Array On-chip Biosensor for the Detection of E. coli O157:H7 Pathogenic Bacteria.- DNA Analysis with a Photo-Diode Array Sensor.- Miniaturized and Integrated Fluorescence Detectors for Microfluidic Capillary Electrophoresis Devices.- Photomultiplier Tubes in Biosensors.- Integrating Waveguide Biosensor.- Detection of Fluorescence Generated in Microfluidic Channel Using In-Fiber Grooves and In-Fiber Microchannel Sensors.- Multiplex Integrating Waveguide Sensor: Signalyte™-II.- CCD Based Fiber-Optic Spectrometer Detection.

Editorial Reviews

From the reviews:

"This is a well written, comprehensive and up-to-date, user-friendly guide to essential information about direct and indirect optical detectors … . the book is written for engineering, chemical and physical science researchers who are developing biosensing technologies, as well as biologists and clinicians. It is also useful as a teaching tool for bioengineering, biomedical engineering, and biology faculty and students. … The clarity of the explanations of important and complex topics and the user friendly quality of the book make it unique." (Omer Iqbal, Doody’s Review Service, February, 2009)

From the Publisher

Reviewer: Omer M. Iqbal, MD, FACC, FESC (Loyola University Medical Center)
Description: This is a well written, comprehensive and up-to-date, user-friendly guide to essential information about direct and indirect optical detectors as well as surface plasmon resonance, interferometric sensors, and other related technologies. This is volume one of a two-volume set.
Purpose: The primary purpose is to provide more access to the technical methods involved in using a variety of biosensors to facilitate decision making.
Audience: According to the authors, the book is written for engineering, chemical, and physical science researchers who are developing biosensing technologies, as well as biologists and clinicians. It is also useful as a teaching tool for bioengineering, biomedical engineering, and biology faculty and students. The authors are recognized authorities in this field.
Features: The book is divided into two parts: part I deals with direct optical detectors and part II deals with indirect optical detectors. The 10 chapters in the first part cover the most common approach for direct optical detection based on evanescent wave physics, especially the most common technology, the surface plasmon resonance. Several other chapters deal with biosensors based on surface plasmon resonance. Other chapters discuss other important optical direct detection methods including resonant mirror, optical ring resonator, interferometric sensors and grating coupler. Part II is divided into 15 chapters and describes various indirect optical detectors designed to measure fluorescence, optical density, changes in color, and chemiluminescence. Other chapters cover the various ways to measure optical signals, such as CCD-based detectors, photodiodes, photomultipliers, and spectrometers. Chapters are well illustrated with figures, graphs, flow diagrams, and photographs. Chapter features readers may find useful are the summaries with key words, the consistent format of introduction, materials, methods, and essential notes, and the adequate list of up-to-date references. Although the book is well illustrated, the use of color in some figures may have enhanced clarity and attractiveness.
Assessment: This well written book provides essential information. The clarity of the explanations of important and complex topics and the user friendly quality of the book make it unique.

Doody's Review Service