Biosensors and Biodetection: Methods and Protocols Volume 2: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors / Edition 1

by Avraham Rasooly, Keith Herold

Overview

Biosensors combine biological recognition elements and signal conversion elements into a biodetection system. They have been developed for a wide variety of biodetection applications, offering the advantages of increased speed and ease of use compared to traditional detection methods. In Biosensors and Biodetection: Methods and Prools, leading experts describe the major technologies in the field in extensive technical detail, allowing readers both to understand the technology and to construct similar devices. Volume 2: Electrochemical and Mechanical Detectors, Lateral Flow and Ligands for Biosensors focuses on direct measurement sensors, indirect methods, ligands, and related technologies, including methods involving electrochemical detectors, recognition ligands, antibodies, aptamers, and peptides, amongst many other subjects. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials, step-by-step, readily reproducible prools, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Biosensors and Biodetection: Methods and Prools is an ideal, user-friendly guide to this vital, versatile technology and a perfect tool for those who wish to further the field.

Product Details

ISBN-13:	9781617379123
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/19/2010
Series:	Methods in Molecular Biology , #504
Edition description:	Softcover reprint of hardcover 1st ed. 2009
Pages:	464
Product dimensions:	7.60(w) x 10.24(h) x 0.04(d)

Table of Contents

Mechanical Detectors.- A Set of Piezoelectric Biosensors Using Cholinesterases.- Piezoelectric Biosensors for Aptamer—Protein Interaction.- Piezoelectric Quartz Crystal Resonators Applied for Immunosensing and Affinity Interaction Studies.- Biosensors Based on Cantilevers.- Piezoelectric-Excited Millimeter-Sized Cantilever Biosensors.- Electrochemical Detectors.- Preparation of Screen-Printed Electrochemical Immunosensors for Estradiol, and Their Application in Biological Fluids.- Electrochemical DNA Biosensors: Prools for Intercalator-Based Detection of Hybridization in Solution and at the Surface.- Electrochemical Biosensor Technology: Application to Pesticide Detection.- Electrochemical Detection of DNA Hybridization Using Micro and Nanoparticles.- Electrochemical Immunosensing Using Micro and Nanoparticles.- Methods for the Preparation of Electrochemical Composite Biosensors Based on Gold Nanoparticles.- Lateral Flow.- Immunochromatographic Lateral Flow Strip Tests.- Liposome-Enhanced Lateral-Flow Assays for the Sandwich-Hybridization Detection of RNA.- Rapid Prototyping of Lateral Flow Assays.- Lateral Flow Colloidal Gold-Based Immunoassay for Pesticide.- Ligands.- Synthesis of a Virus Electrode for Measurement of Prostate Specific Membrane Antigen.- In Vivo Bacteriophage Display for the Discovery of Novel Peptide-Based Tumor-Targeting Agents.- Biopanning of Phage Displayed Peptide Libraries for the Isolation of Cell-Specific Ligands.- Biosensor Detection Systems: Engineering Stable, High-Affinity Bioreceptors by Yeast Surface Display.- Antibody Affinity Optimization Using Yeast Cell Surface Display.- Using RNA Aptamers and the Proximity Ligation Assay for the Detection of Cell Surface Antigens.- In Vitro Selection of Protein-Binding DNA Aptamers as Ligandsfor Biosensing Applications.- Protein and DNA Preparation.- Immobilization of Biomolecules onto Silica and Silica-Based Surfaces for Use in Planar Array Biosensors.- Rapid DNA Amplification Using a Battery-Powered Thin-Film Resistive Thermocycler.

Editorial Reviews

Reviewer: Omer M. Iqbal, MD, FACC, FESC (Loyola University Medical Center)
Description: This well written, user friendly book describes electrochemical and mechanical detectors, lateral flow, and ligands for biosensors.
Purpose: According to the authors, the primary aim of the book is to describe the basic types and basic elements of biosensors from a methods point of view by representing major technologies in the field with enough technical details so that informed readers can both understand the technology and be able to build similar devices.
Audience: The audience includes engineering, chemical, and physical science researchers, who are developing biosensing technologies. Other intended audiences are biologists and clinicians who are users and developers of applications for the technologies. This book may also serve as a teaching tool for bioengineering, biomedical engineering, and biology faculty and students.
Features: The five chapters in part I on direct measurement sensors describe several mechanical detectors that modify their mechanical properties as a result of biological interactions such as piezoelectric biosensors and cantilever-based systems. Parts II and III deal with indirect methods. The six chapters in part II deal with several electrochemical detectors, including the most commonly used biosensors for glucose monitoring. The four chapters in part III cover lateral flow technologies involving lateral flow assays such as simple immunonodetection devices used mainly for medical diagnostics, including laboratory, home, and point-of-care detection, a common format being a dipstick. The seven chapters in part IV describe recognition ligands, including antibodies, aptamers, and peptides, which are key elements in any biosensor. The final part has two chapters on protein and DNA preparations. Numerous figures, graphs, flow charts, and photographs are used throughout the book. Each chapter begins with a summary, uses a format of introduction, materials, methods, and notes, and concludes with up-to-date references for further reading. Color figures may have enhanced comprehension of the complex topics.
Assessment: This second volume of Biosensors and Biodetection is well written and well illustrated. Readers will find it very useful.

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