Basic Cloning Procedures

by Valdis Berzins (Editor)

Overview

This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The prools include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each prool includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.

Product Details

ISBN-13:	9783642489778
Publisher:	Springer Berlin Heidelberg
Publication date:	07/07/2012
Series:	Springer Lab Manuals
Edition description:	1998
Pages:	169
Product dimensions:	6.10(w) x 9.25(h) x 0.01(d)

Table of Contents

1 cDNA Synthesis and Cloning.- 2 DNA Sequencing.- 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.- 4 PCR-Based Site-Specific Mutagenesis.- 5 Analysis of Specific Protein-DNA Interactions.- 6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.- 7 Immunological Methods for Analysis of Recombinant Proteins.- 8 Protein Synthesis in Cell-Free Systems.