Baculovirus and Insect Cell Expression Protocols / Edition 2

by David W. Murhammer

Overview

Baculovirus Expression Prools is a detailed guide for using the baculovirus expression vector system (BEVS) and/or insect cells to produce recombinant proteins. Procedures for the development and production of baculovirus insecticides at both laboratory and large scale are described in the kind of step-by-step format that the Methods in Molecular Biology™ series helped standardize. This book is a one-stop source for information on baculoviruses, and includes sections on using modified baculoviruses to express genes in mammalian cells, using Drosophila cell lines, isolating new cell lines, and developing serum-free medium. Specific applications, including using baculovirus and insect cell system to study apoptosis, are also discussed. This updated and expanded edition of Baculovirus Expression Prools provides a perfect lab manual for biochemists, molecular biologists, biochemical engineers, and others for using the BEVS or insect cell lines to produce recombinant proteins.

Product Details

ISBN-13:	9781617376290
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/19/2010
Series:	Methods in Molecular Biology , #388
Edition description:	Softcover reprint of hardcover 2nd ed. 2007
Pages:	470
Product dimensions:	5.98(w) x 9.02(h) x 0.35(d)

Table of Contents

Useful Tips, Widely Used Techniques, and Quantifying Cell Metabolic Behavior.- Baculovirus Molecular Biology/Development of Recombinant Baculoviruses.- to Baculovirus Molecular Biology.- Baculovirus Transfer Vectors.- Recombinant Baculovirus Isolation.- Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System.- Insect Cell Culture.- Available Lepidopteran Insect Cell Lines.- Lepidopteran Insect Cell Line Isolation from Insect Tissue.- Development of Serum-Free Media for Lepidopteran Insect Cell Lines.- Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses.- Protein Production with Recombinant Baculoviruses.- Small-Scale Protein Production With the Baculovirus Expression Vector System.- Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System.- Production of Baculovirus-Expressed Recombinant Proteins in Wave Bioreactors.- Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae.- Virus-like Particle and Viral Vector Production Using the Baculovirus Expression Vector System/Insect Cell System.- Recombinant Protein Production with Transformed Insect Cells.- Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression.- Transformation of Drosophila Cell Lines.- Transforming Lepidopteran Insect Cells for Improved Protein Processing.- Baculovirus Development and Production for Use as Insecticides.- to the Use of Baculoviruses as Biological Insecticides.- Baculovirus Insecticide Production in Insect Larvae.- Evaluation of the Insecticidal Efficacy of Wild-Type and Recombinant Baculoviruses.- Miscellaneous Techniques and Applications of the Baculovirus/Insect Cell System.- Monitoring and Visualization of Baculovirus Infection UsingGreen Fluorescent Protein Strategy.- Alternative Bioreactor Strategy for Probing Infection and Production.- Methods for Gene Silencing With RNAi.- Using the Baculovirus/Insect Cell System to Study Apoptosis.- Generation of Envelope-Modified Baculoviruses for Gene Delivery Into Mammalian Cells.