Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration / Edition 1

Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration / Edition 1

ISBN-10:
0470543981
ISBN-13:
9780470543986
Pub. Date:
08/09/2010
Publisher:
Wiley
ISBN-10:
0470543981
ISBN-13:
9780470543986
Pub. Date:
08/09/2010
Publisher:
Wiley
Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration / Edition 1

Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration / Edition 1

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Overview

A much-needed primer on the use of laser flow cytometry for stem cell analysis

Laser flow cytometry is a powerful tool for rapid analysis of cells for marker expression, cell cycle position, proliferation, and apoptosis. However, no resources specifically address the use of this methodology for the study of stem cells; this is especially important as stem cell analysis involves specialized methods and staining procedures based on specific characteristics such as marker expression, cell size, drug transport, and efflux of the stem cells.

Now, this book reviews these procedures, discusses the science behind them, and provides real-world examples to illustrate the usefulness of the methods. It brings together world-class experts in pathology, biophysics, immunology, and stem cell research, who draw upon their extensive experience with the methods and show examples of good data to help guide researchers in the right direction.

Chapter coverage includes:

  • Stem cell analysis and sorting using side population
  • Flow cytometry in the study of proliferation and apoptosis
  • Stem cell biology and application
  • Identification and isolation of very small embryonic-like stem cells from murine and human specimens
  • Hematopoietic stem cells—issues in enumeration
  • Human embryonic stem cells: long-term culture and cardiovascular differentiation
  • Limbal stem cells and corneal regeneration
  • Flow cytometric sorting of spermatogonial stem cells
  • Breast cancer stem cells
  • Stem cell marker expression in cells from body cavity fluids

This book is an essential resource for all graduate students, practitioners in developing countries, libraries and book repositories of universities and research institutions, and individual researchers. It is also of interest to laboratories engaged in stem cell research and use of stem cells for tissue regeneration, and to any organization dealing in stem cell and tissue regeneration research.


Product Details

ISBN-13: 9780470543986
Publisher: Wiley
Publication date: 08/09/2010
Pages: 410
Product dimensions: 7.20(w) x 10.00(h) x 0.80(d)

About the Author

Awtar Krishan, Ph.D is a Professor of Pathology at the University of Miami School of Medicine. From 1965 to 1977, he was at the Sidney Farber Cancer Institute, Harvard MedicalSchool as Head of the Tissue Ultrastructure and Experimental Pathology Labs. Some of his recent work has focused on development and applications of a new flow cytometer which can measure cell volume and marker expression of stem cells in human apheresis samples. Dr. Krishan has more than 160 publications in peer reviewed journals.

H. Krishnamurthy is working as Scientific Officer 'E' and in charge of Flow Cytometry and Confocal Microscopy Facilities at the National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bangalore, India. His research interest is hormonal regulation of spermatogenesis and trafficking and signaling of gonadotropin receptors.

Satish Totey is Chief Scientific Officer, Stempeutics Research Pvt. Ltd and Director at the Manipal Institute of Regenerative Medicine inBangalore. Dr. Totey was amongst the small number of researchers who pioneered the isolation and characterization of pluripotent stem cells from human embryos. The cell lines he established have been listed in the NIH stem cell registry. He has also developed up-scaling of mesenchymal stem cells and is currently conducting phase-I and II clinical trials for myocardial infarction, leg ischemia, cerebral stroke and Multiple Sclerosis. He has published 90 research papers and holds 12US patents.

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Table of Contents

Contributors vii

Preface xi

1 Basics of Flow Cytometry H. Krishnamurthy L. Scott Cram 1

2 Practical Considerations for Flow Cytometric Sorting of Stem Cells Geoffrey W. Osborne 13

3 Stem Cell Analysis and Sorting Using Side Population Analysis William Telford 25

4 Flow Cytometry in the Study of Proliferation and Apoptosis Michael G. Ormerod Ronald M. Hamelik 45

5 Flow Cytometric Analysis of Drug Transport and Efflux in Stem Cells Awtar Krishan Ronald M. Hamelik 61

6 Stem Cell Biology and Application Swapnil Totey Rajarshi Pal Murali Krishna Mamidi Vijayendran Govindasamy Satish Totey 75

7 Identification and Isolation of Very Small Embryonic-like Stem Cells from Murine and Human Specimens Ewa K. Zuba-Surma Dong-Myung Shin Izabella Klich Janina Ratajczak Magda Kucia Mariusz Z. Ratajczak 91

8 Electronic Volume of Hematopoietic Stem Cells Siddharth Sharma Awtar Krishan 103

9 Hematopoietic Stem Cells: Issues in Enumeration Michael Keeney D. Robert Sutherland 115

10 Embryonic Stem Cells: Development and Characterization Vijay Bhaskar R. Konala Villoo Morawala-Patell Aparna Khanna 135

11 Human Embryonic Stem Cells: Long-Term Culture and Cardiovascular Differentiation Maneesha Inamdar 161

12 Mesenchymal Stromal Cells and Their Clinical Applications Jyoti Kode Vivek Tanavde 175

13 Circulating Adult Stem Cells of Hematopoietic Origin for Vascular and Neural Regeneration Lissy K. Krishnan 189

14 Flow Cytometric Characterization of Neural Progenitors Derived from Human Pluripotent Stem Cells Raj R. Rao Sujoy K. Dhara Shilpa Iyer David W. Machacek 211

15 Limbal Stem Cells and Corneal Regeneration Geeta K. Vemuganti Murali Mohan Sagar Balla Shubha Tiwari 223

16 Flow Cytometric Sorting of Spermatogonial Stem Cells B. S. Srinag J. M. Kalappurakkal G.H. Mohan H. Krishnamurthy 241

17 Breast Cancer Stem Cells Devaveena Dey Annapoorni Rangarajan 251

18 Tumor Stem Cell Marker Expression in Cells from Body Cavity Fluids Awtar Krishan Deepti Sharma Ronald M. Hamelik 271

Index 279

What People are Saying About This

From the Publisher

"Contributors from a wide range of medical and biological sciences explain methods and procedures of flow cytometry specific to its use in research on stem cells and related matters." (SciTech Book News, December 2010)

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