Antibody Engineering: A Practical Approach / Edition 1

by John McCafferty, Hennie R. Hoogenboom, David J. Chiswell

Overview

Recombinant DNA techniques have revolutionized the isolation and production of antibodies in recent years. This has resulted in rapid changes in how to handle antibodies for research. This new Practical Approach volume responds to this change by assembling, in one volume, protocols which allow the researcher to isolate a new antibody, analyse its properties, format the right antibody molecule or fragment, and produce it in usable quantities. The book is divided into two parts: the first describes the generation and analysis of antibodies and the second covers engineering and production. This timely book will be of interest to those involved in both clinical and molecular biological applications of antibodies.

Product Details

ISBN-13:	9780199635924
Publisher:	Oxford University Press
Publication date:	08/29/1996
Series:	Practical Approach Series , #169
Edition description:	1st ed
Pages:	348
Product dimensions:	6.13(w) x 9.19(h) x 0.73(d)

About the Author

McCafferty, John; Hoogenboom, Hennie; Chiswell, Dave

The contributors represent the specialties of biochemistry, molecular genetics, molecular oncology, and biology. Most are from research institutes and universities in the U.S., the U.K., France, Germany, Switzerland, and Norway. Institutions prominently represented include Ludwig Institute for Cancer Research, Institut Pasteur, Cambridge Antibody Technology, Ltd., Universitat Zurich, and Beckman Research Institute of the City of Hope.

Table of Contents

1. Construction and use of antibody gene repertoires2. Affinity maturation of antibodies using phage display3. Human antibody repertoires in transgenic mice: manupulation and transfer of YACs4. Measuring antibody affinities in solution5. Measuring antibody affinities using Bio-sensors6. Analysis of human antibody sequences7. Rodent to human antibodies by CDR grafting8. Rodent to human antibodies by phage display9. Choosing and manipulating effector functions10. Secretion and purification of antibody fragments expressed in bacteria11. Production of single chain Fv monomers and multimers12. Expression of immunoglobulin genes in mammalian cells13. In vitro linkage of antibody fragments into dimeric and multimeric forms

Editorial Reviews

"This resource is highly recommended for libraries and bookstores of academic, medical technology, and pharmaceutical institutions."—Doody's Health Sciences Book Review Home Page

From the Publisher

5 Stars from Doody

This volume of The Practical Approach Series provides strategies and up-to-date protocols for the design and production of monoclonal antibodies using recombinant DNA technology. Topics include analysis and construction of antibody gene repertoires; transfer and manipulation of human antibody repertoires in transgenic mice; expression of immunoglobulin genes in mammalian cells; large-scale production of antibody fragments in prokaryotes; conversion of rodent into human antibodies by CDR grafting and guided selection; measurement and manipulation of antibody affinities; and optimization of antibody effector functions. The book is an indispensable manual for researchers wishing to improve the qualities of monoclonal antibodies for specific research applications and for those who seek advanced and proven methods to generate high-affinity, high-specificity human antibodies for in vivo therapeutic use. Written by internationally recognized experts in the field, each chapter contains sufficient background information explaining the principles of pertinent methodological approaches and provides up-to-date, fully tested, step-by-step protocols. These are supplemented with useful germline and primer sequence information and extensive referencing. This resource is highly recommended for libraries and bookstores of academic, medical technology, and pharmaceutical institutions.

Katalin Mikecz

Reviewer: Katalin Mikecz, MD, PhD (Rush Medical College of Rush University)
Description: This volume of The Practical Approach Series provides strategies and up-to-date protocols for the design and production of monoclonal antibodies using recombinant DNA technology.
Purpose: Topics include analysis and construction of antibody gene repertoires; transfer and manipulation of human antibody repertoires in transgenic mice; expression of immunoglobulin genes in mammalian cells; large-scale production of antibody fragments in prokaryotes; conversion of rodent into human antibodies by CDR grafting and guided selection; measurement and manipulation of antibody affinities; and optimization of antibody effector functions.
Audience: The book is an indispensable manual for researchers wishing to improve the qualities of monoclonal antibodies for specific research applications and for those who seek advanced and proven methods to generate high-affinity, high-specificity human antibodies for in vivo therapeutic use.
Features: Written by internationally recognized experts in the field, each chapter contains sufficient background information explaining the principles of pertinent methodological approaches and provides up-to-date, fully tested, step-by-step protocols. These are supplemented with useful germline and primer sequence information and extensive referencing.
Assessment: This resource is highly recommended for libraries and bookstores of academic, medical technology, and pharmaceutical institutions.

Doody's Review Service