Advanced Flow Cytometry: Applications in Biological Research

by R.C. Sobti (Editor), A. Krishan (Editor)

Overview

Flow cytometry has rapidly evolved into a technique for rapid analysis of DNA content, cellular marker expression and electronic sorting of cells of interest for further investigations. Flow cytometers are being extensively used for monitoring of cellular DNA content, phenotype expression, drug transport, calcium flux, proliferation and apoptosis. Phenotypic analysis of marker expression in leukemic cells has become an important tool for diagnostic and therapeutic monitoring of patients. Recent studies have explored the use of flow cytometry for monitoring hormone receptor expression in human solid tumors and for studies in human genomics. Contributions in the current volume are based on presentations made at the First Indo-US workshop on Flow Cytometry in which experts from USA, UK and India discussed applications of flow cytometry in biological and medical research. This book will be of interest to post graduates and researchers in the fields of pathology, cytology, cell biology and molecular biology.

Product Details

ISBN-13:	9789401706230
Publisher:	Springer-Verlag New York, LLC
Publication date:	04/17/2013
Sold by:	Barnes & Noble
Format:	eBook
File size:	7 MB

Table of Contents

Flow cytometry, an overview.- High resolution flow cytometric analysis of electronic nuclear volume and DNA content in normal and abnormal human tissue.- Analysis of heterogeneous red cell populations by flow cytometry.- Chromosome sorting and genomics.- Analysis of intracellular cytokines using flowcytometry.- Cell array coupled with laser scanning cytometry allows easy analysis of changes in cyclin expression during the cell cycle.- Flow cytometry and its application in small animal oncology.- Flow cytometric monitoring of drug resistance in human tumor cells.- Androgen receptor expression in archival human breast tumors.- Membrane oxidative damage and apoptosis in cervical carcinoma cells of patients after radiation therapy.- Nitric oxide mediated modulation of free radical generation response in the rat polymorphonuclear leukocytes: A flowcytometric study.- Flowcytometric detection of PNH defect and response to therapy in aplastic anemia patients.- Host-pathogen interactions: leukocyte phagocytosis and associated sequelae.- Flow cytometric techniques for the detection of microorganisms.- A flow cytometric approach to assess phytoplankton respiration.- Cellular and serological markers of disease activity in Indian patients with HIV/AIDS.

Editorial Reviews

Reviewer: Valerie L Ng, PhD MD(Alameda County Medical Center/Highland Hospital)
Description: This thin hardcover book is a compilation of presentations made at the first Indo-U.S. Workshop on Flow Cytometry and is a reprint of the original publication (Methods in Cell Science, Volume 24, Nos. 1-3, 2003).
Purpose: The purpose of this book is to educate the reader on the recent advances and many diverse and broad applications of flow cytometry.
Audience: This book would be useful for those working in "core" flow cytometry facilities, as well as anyone interested in its applications to his or her area of scientific investigation.
Features: This book contains 16 sections, most of which are original articles. The first section is a broad overview of the basic principles of flow cytometry. The next three are basic methodological articles applicable to many scientific areas (e.g., red cell analysis, chromosome sorting and genomics, cytokine analysis). The article on chromosome sorting and genomics is helpful in that it includes buffer recipes for chromosome preparations and staining. The subsequent 12 articles are specific and focused applications of flow cytometry to a broad array of scientific questions. There's undoubtedly an application for everyone in this book. The final article dealing with flow cytometric methods to monitor HIV/AIDS is especially interesting in that it critically analyzes and recommends a subset of various surrogate markers - not all of which relied on flow cytometry — for progression of HIV disease.
Assessment: This book is by no means a comprehensive reference of flow cytometry, but a snapshot of its technological capabilities as of 2002. I'd recommend it for those interested in the technology of flow cytometry and learning about its evolving and new applications.

Doody's Review Service

"This book will be of interest to postgraduates and researchers in the fields of pathology, cytology, cell biology, and molecular biology."

(Journal of Investigational Allergology and Clinical Immunology, 13:4 (2003)

From the Publisher