Adenoviral Vectors for Gene Therapy / Edition 2

by David T. Curiel

Overview

Adenoviral Vectors for Gene Therapy, Second Edition provides detailed, comprehensive coverage of the gene delivery vehicles that are based on the adenovirus that is emerging as an important tool in gene therapy. These exciting new therapeutic agents have great potential for the treatment of disease, making gene therapy a fast-growing field for research.

This book presents topics ranging from the basic biology of adenoviruses, through the construction and purification of adenoviral vectors, cutting-edge vectorology, and the use of adenoviral vectors in preclinical animal models, with final consideration of the regulatory issues surrounding human clinical gene therapy trials. This broad scope of information provides a solid overview of the field, allowing the reader to gain a complete understanding of the development and use of adenoviral vectors.

Product Details

ISBN-13:	9780128002766
Publisher:	Elsevier Science
Publication date:	04/06/2016
Edition description:	2nd ed.
Pages:	868
Product dimensions:	6.00(w) x 9.00(h) x (d)

About the Author

David T. Curiel, M.D., Ph.D. is the Director of the Cancer Biology Division of the Department of Radiation Oncology at Washington University School of Medicine. Dr. Curiel graduated medical school at Emory University in 1982, where he also completed his internship and residency in Internal Medicine. Dr. Curiel’s scientific training includes tenureship at the National Institutes of Health in Bethesda, Maryland at the Pulmonary Branch of the Heart and Lung, and Blood Institute (NHLBI) from 1985-1989, and a fellowship in Biotechnology at the National Cancer Institute, Navy Medical Oncology Branch from 1989-1990. He received his Ph.D. from University of Groningen in The Netherlands in 2002. Dr. Curiel has been at Washington University School of Medicine since 2011. In addition to his role as Director of the Cancer Biology Division, he is Director of the Biologic Therapeutics Center.

Table of Contents

1. Adenovirus Structure 2. Biology of Adenovirus Cell Entry: Receptors, Pathways, Mechanisms 3. Adenovirus Replication 4. Adenoviral Vector Construction I: Mammalian Systems 5. Adenoviral Vector Construction II: Bacterial Systems 6. Upstream Bioprocess for Adenovirus Vectors 7. Propagation of Adenoviral Vectors: Use of PER.C6™ Cells 8. Purification of Adenovirus 9. Targeted Adenoviral Vectors I: Transductional Targeting 10. Targeted Adenoviral Vectors III: Transcriptional Targeting 11. Adenoviral Vector Targeting via Mitigation of Liver Sequestration 12. Molecular Design of Oncolytic Adenoviruses 13. Conditionally Replicative Adenoviruses—Clinical Trials 14. Innate Immune Response to Adenovirus Vector Administration In Vivo 15. Antibodies against Adenoviruses 16. Methods to Mitigate Immune Responses to Adenoviral Vectors 17. Helper-Dependent Adenoviral Vectors 18. Hybrid Adenoviral Vectors 19. Xenogenic Adenoviral Vectors 20. Engineering Chimeric Adenoviruses: Exploiting Virus Diversity for Improved Vectors, Vaccines, and Oncolytics 1. Introduction 22. Adenoviral Vectors Vaccine: Capsid Incorporation of Antigen 23. Utility of Adenoviral Vectors in Animal Models of Human Disease I: Cancer 24. In Situ Vaccination with Adenoviral Vectors to Treat Cancer 25. Utility of Adenoviral Vectors in Animal Models of Human Disease II: Genetic Disease 26. Adenoviral Vectors for Pulmonary Disease (Pulmonary Vascular Disease) 27. Utility of Adenoviral Vectors in Animal Models of Human Disease III: Acquired Diseases 28. Animal Models of Gene Therapy for Cardiovascular Disease 29. Polymer-Anchored Adenovirus as a Therapeutic Agent for Cancer Gene Therapy 30. Adenoviral Vectors for RNAi Delivery 31. Imaging and Adenoviral Gene Therapy 32. Regulation of Adenoviral Vector-Based Therapies: An FDA Perspective

What People are Saying About This

From the Publisher

This detailed, comprehensive, fully updated edition provides the latest coverage of the gene delivery vehicles that are based on the adenovirus that is emerging as an important tool in gene therapy, highlighting their potential uses for the treatment of disease that includes information on their construction, propagation, and purification of adenoviral vectors

Editorial Reviews

3 Stars from Doody

Reviewer: Daniel G. Miller, MD, PhD (University of Washington School of Medicine)
Description: This is a comprehensive book outlining adenovirus structure, lifecycle, and construction of vectors for efficient gene transfer.
Purpose: The book reviews an extensive body of literature and quickly brings scientists up-to-date in a rapidly progressing field of research. It serves as an introductory book that quickly proceeds to the latest developments in adenovirus vector gene therapy, as well as a reference to those wanting to keep important advances in the field at their fingertips.
Audience: The book will be most useful to scientific investigators involved in adenovirus gene therapy research or basic adenovirus biology.
Features: The book covers the basics of adenovirus biology including adenovirus structure, receptor interaction, internalization, intracellular uncoating of virus particles, and replication. Authors cover vector construction, purification, cell targeting, and tissue specific gene expression in the subsequent chapters. The mammalian immune response to adenovirus vectors is addressed as well, and construction of vectors that minimize antigenicity is reviewed as a solution to this problem. Finally, there are several sections on attenuated replication competent adenovirus vectors and their use in targeting cancer cells for destruction. The most unique section of the book is the discussion of the utility of adenovirus vectors for the treatment of human genetic and acquired disease. One chapter on testing Adenoviral gene transfer products and FDA expectations is particularly useful to investigators designing new vectors with the goal of human clinical trials. The book does not contain an index, but the table of contents is listed in a detailed outline format, allowing the reader to quickly find the section of interest.
Assessment: This is a well organized collection of reviews written by authorities in the field that covers key aspects in the use of adenovirus as a gene transfer vehicle. The complete coverage of so many different aspects of adenovirus vector biology will be valuable to scientists beginning or well established in this rapidly changing field.

Doody's Review Service

"This is a well organized collection of reviews written by authorities in the field that covers key aspects in the use of adenovirus as a gene transfer vehicle. The complete coverage of so many different aspects of adenovirus vector biology will be invaluable to scientists beginning or well established in this rapidly changing field." —DOODY'S (March 2003)

From the Publisher