3D Cell Culture: Methods and Protocols / Edition 1

by John W. Haycock

Overview

Developed for a range of tissues where the culture environment takes into account the spatial organization of the cells therein, 3D cell culture models serve to bridge the gap between in vivo studies at one extreme with that of simple cell monolayers at the other. In 3D Cell Culture: Methods and Prools, international experts describe a number of basic and applied methodologies taken from a breadth of scientific and engineering disciplines, many of which deal with direct applications of 3D culture models, most notably in the formation of tissues for clinical purpose. Beginning with an overview of the biological and materials scaffold requirements for successfully creating 3D models, the book delves into topics such as general scaffold design and fabrication techniques, models for bone, skin, cartilage, nerve, bladder, and hair follicles, and chapters on bioreactor design, imaging, and stem cells. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory prools, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, 3D Cell Culture: Methods and Prools serves as a basic manual for laboratory-based scientists who not only need to have a comprehensive range of techniques contained within a single text but also require techniques described using a standard, convenient format.

Product Details

ISBN-13:	9781607619833
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/02/2010
Series:	Methods in Molecular Biology , #695
Edition description:	2011
Pages:	343
Product dimensions:	7.20(w) x 10.10(h) x 1.00(d)

Table of Contents

Preface v

Contributors ix

1 3D Cell Culture: A Review of Current Approaches and Techniques John W. Haycock 1

2 Scaffolds for Tissue Engineering and 3D Cell Culture Eleonora Carletti Antonella Motta Claudio Migliaresi 17

3 Tracking Nanoparticles in Three-Dimensional Tissue-Engineered Models Using Confocal Laser Scanning Microscopy Vanessa Hearnden Sheila MacNeil Giuseppe Battaglia 41

4 Using Immuno-Scanning Electron Microscopy for the Observation of Focal Adhesion-substratum interactions at the Nano- and Microscale in S-Phase Cells ManusJ.P. Biggs R. Geoff Richards Matthew J. Dalby 53

5 3D Sample Preparation for Orthopaedic Tissue Engineering Bioreactors Sarah H. Cartmell Sarah Rathbone Gemma Jones L. Araida Hidalgo-Bastida 61

6 Quantification of mRNA Using Real-Time PCR and Western Blot Analysis of MAPK Events in Chondrocyte/Agarose Constructs David A. Lee June Brand Donald Salter Oto-Ola Akanji Tina T. Chowdhury 77

7 Genetic Modification of Chondrocytes Using Viral Vectors Teresa Coughlan Aileen Crawford Paul Hatton Michael Barker 99

8 Stem Cell and Neuron Co-cultures for the Study of Nerve Regeneration Paul J. Kingham Cristina Mantovani Giorgio Terenghi 115

9 Production of Tissue-Engineered Skin and Oral Mucosa for Clinical and Experimental Use Sheila MacNeil Joanna Shepherd Louise Smith 129

10 Three-Dimensional Alignment of Schwann Cells Using Hydrolysable Microfiber Scaffolds: Strategies for Peripheral Nerve Repair Celia Murray-Dunning Sally L. McArthur Tao Sun Rob McKean Anthony J. Ryan John W. Haycock 155

11 Encapsulation of Human Articular Chondrocytes into 3D Hydrogel: Phenotype and Genotype Characterization Rui C. Pereira Chiara Gentili Ranieri Cancedda Helena S. Azevedo Rui L. Reis 167

12 Micro-structured Materials and Mechanical Cues in 3D Collagen Gels James B. Phillips Robert Brown 183

13 Organotypic and 3D Reconstructed Cultures of the Human Bladder and Urinary Tract Claire L. Varley Jennifer Southgate 197

14 Ex Vivo Organ Culture of Human Hair Follicles: A Model Epithelial-Neuroectodermal-Mesenchymal Interaction System Desmond J. Tobin 213

15 Human Endothelial and Osteoblast Co-cultures on 3D Biomaterials Ronald E. Unger Sven Halstenberg Anne Sartoris C. James Kirkpatrick 229

16 Assessment of Nanomaterials Cytotoxicity and Internalization Noha M. Zaki Nicola Tirelli 243

17 Practical Aspects of OCT Imaging in Tissue Engineering Stephen J. Matcher 261

18 Osteogenic Differentiation of Embryonic Stem Cells in 2D and 3D Culture Lee Buttery Robert Bielby Daniel Howard Kevin Shakesheff 281

19 3D Structuring of Biocompatible and Biodegradable Polymers Via Stereolithography Andrew A. Gill Frederik Claeyssens 309

20 Alvetex®: Polystyrene Scaffold Technology for Routine Three Dimensional Cell Culture Eleanor Knight Bridgid Murray Ross Carnachan Stefan Przyborski 323

Index 341

Editorial Reviews

From the reviews:
“This book is an excellent resource to help researchers find practical information to enable them to develop three-dimensional cell culture systems. … anyone who wishes to enter this field should be able to use this book to develop their own bespoke 3D system. In conclusion, I would recommend this book as being very useful to a wide audience … . will be essential for graduate students and postdocs as well as to investigators wishing to expand their work into 3D cell culture and tissue engineering.” (Julian B. Chaudhuri, ChemBioChem, Vol. 12, 2011)
“A necessary book for all who use cell culture. No other words can better tell the reader about this compendium of the most innovative culture techniques we hold to try to reach the ultimate goal of cell culture … . For those who wish to enter the field, some introductory reviews explain the present-day challenges in 3D culture while more detailed prools talk about bioreactors and agarose constructs.” (Carlo Alberto Redi, European Journal of Hishemistry, Vol. 55, 2011)

From the Publisher